New_variants : ORF1a


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Key Findings
3 significant mutations were identified in the spike protein (L452R, W152C and S13I) of the California variant B.1.427/B.1.429 with no particular difference from non-variant lineages (B.1.427, B.1.429, other Clade 20C lineages).
PMID 33991487
Title Transmission, infectivity, and neutralization of a spike L452R SARS-CoV-2 variant
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Date of publishing2021 Apr 20

Sequence : ORF1a


Total row(s): 2
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Key Findings
Primer sets for Orf1a gene used in nested RT-PCR for 1st round of PCR.
PMID 32074516
Title Development of genetic diagnostoc methods for detection for Novel Coronavirus 2019 (nCoV-2019) in Japan
Author(s) nameShirato K, Nao N, Katano H, Takayama I, Saito S, Kato F, Katoh H, Sakata M, Nakatsu Y, Mori Y, Kageyama T, Matsuyama S, Takeda M.
Journal Jpn J Infect Dis
Impact factor1.11
Citation count72
Date of publishing2020 Jul 22
Primer sets for Orf1a gene used in nested RT-PCR for 2nd round of PCR.
PMID 32074516
Title Development of genetic diagnostoc methods for detection for Novel Coronavirus 2019 (nCoV-2019) in Japan
Author(s) nameShirato K, Nao N, Katano H, Takayama I, Saito S, Kato F, Katoh H, Sakata M, Nakatsu Y, Mori Y, Kageyama T, Matsuyama S, Takeda M.
Journal Jpn J Infect Dis
Impact factor1.11
Citation count72
Date of publishing2020 Jul 22

Diagnostics : ORF1a


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Key Findings
The implementation of Nested RT-PCR for the detection of COVID-19, based on SARS-CoV-2 Orf1a and S genes.
PMID 32074516
Title Development of genetic diagnostic methods for detection for Novel Coronavirus 2019 (nCoV-2019) in Japan
Author(s) nameShirato K, Nao N, Katano H, Takayama I, Saito S, Kato F, Katoh H, Sakata M, Nakatsu Y, Mori Y, Kageyama T, Matsuyama S, Takeda M.
Journal Jpn J Infect Dis
Impact factor1.11
Citation count72
Date of publishing2020 Jul 22