New_variants : Nucleocapsid

Total row(s): 2
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Key Findings
Original Article
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Novel variant 20E (EU1) was characterized by a spike mutation (A222V) as well as amino acid substitutions in the nucleocapsid protein. However, the mutations did not confer any transmission advantage on the variant. -
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PMID
-
Date of Publishing: 2021 Mar 24
Title Emergence and spread of a SARS-CoV-2 variant through Europe in the summer of 2020
Date of Entry 18_29_59 Jul
Novel variant 20A.EU2 was characterized by amino acid substitutions in the spike region (S447N) and nucleocapsid protein. This variant was predominant in France, Belgium, and Switzerland. -
()
PMID
-
Date of Publishing: 2021 Mar 24
Title Emergence and spread of a SARS-CoV-2 variant through Europe in the summer of 2020
Date of Entry 18_29_59 Jul

Sequence : Nucleocapsid

Total row(s): 17
Select item(s)
Key Findings
Original Article
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62 mutations identified, including 30 mis-sense mutations, in 22 Moroccan patient isolates showed that Spike_D614G and NSP12_P323L mutations were present in all the analyzed sequences, whereas N_G204R and N_R203K were present in 9 sequences. 33558859
(Biosaf Health)
PMID
33558859
Date of Publishing: 2021 Feb 3
Title Genetic diversity and genomic epidemiology of SARS-CoV-2 in Morocco
Author(s) nameBadaoui B, Sadki K et al.
Journal Biosaf Health
Impact factor
Cant find
Citation count: 1
Primer and probe sequence specific for N1 gene(Nucleocapsid) of SARS-CoV-2 used in RT-qPCR assay. 33338082
(PLoS One)
PMID
33338082
Date of Publishing: 2020
Title A simplified SARS-CoV-2 detection protocol for research laboratories
Author(s) namePaz S, Mauer C et al.
Journal PLoS One
Impact factor
2.87
Citation count: 1
Primer and probe sequence specific for N2 gene (Nucleocapsid gene) of SARS-CoV-2 used in RT-qPCR assay. 33338082
(PLoS One)
PMID
33338082
Date of Publishing: 2020
Title A simplified SARS-CoV-2 detection protocol for research laboratories
Author(s) namePaz S, Mauer C et al.
Journal PLoS One
Impact factor
2.87
Citation count: 1
The mutation analysis of SARS-CoV-2 genomes from 13 different countries revealed the country-specific amino acid variations in the nucleocapsid protein. 32881907
(PLoS One)
PMID
32881907
Date of Publishing: 2020
Title Comparative genome analysis of novel coronavirus (SARS-CoV-2) from different geographical locations and the effect of mutations on major target proteins: An in silico insight
Author(s) nameKhan MI, Khan ZA et al.
Journal PLoS One
Impact factor
2.87
Citation count: 7
The second set of primer and probe sequences (YCH-N2) based on the Nucleocapsid gene developed by YCH (Yamanashi Central Hospital), used in real-time RT-PCR assay. 32650037
(J Virol Methods)
PMID
32650037
Date of Publishing: 2020 Oct
Title Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay
Author(s) name Hirotsu Y, Mochizuki H, Omata M.
Journal J Virol Methods
Impact factor
1.76
Citation count: 9
The first set of primer and probe sequences (YCH-N1) based on the Nucleocapsid gene developed by YCH (Yamanashi Central Hospital), used in real-time RT-PCR assay. 32650037
(J Virol Methods)
PMID
32650037
Date of Publishing: 2020 Oct
Title Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay
Author(s) name Hirotsu Y, Mochizuki H, Omata M.
Journal J Virol Methods
Impact factor
1.76
Citation count: 9
The second set of primer and probe sequences (NIID-N2) based on the Nucleocapsid gene developed by NIID, used in real-time RT-PCR assay. 32650037
(J Virol Methods)
PMID
32650037
Date of Publishing: 2020 Oct
Title Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay
Author(s) name Hirotsu Y, Mochizuki H, Omata M.
Journal J Virol Methods
Impact factor
1.76
Citation count: 9
The first set of primer and probe sequences (NIID-N1) based on the Nucleocapsid gene developed by NIID, used in real-time RT-PCR assay. 32650037
(J Virol Methods)
PMID
32650037
Date of Publishing: 2020 Oct
Title Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay
Author(s) name Hirotsu Y, Mochizuki H, Omata M.
Journal J Virol Methods
Impact factor
1.76
Citation count: 9
The third set of primer and probe sequences (CDC-N3) based on the Nucleocapsid gene developed by CDC, used in real-time RT-PCR assay. 32650037
(J Virol Methods)
PMID
32650037
Date of Publishing: 2020 Oct
Title Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay
Author(s) name Hirotsu Y, Mochizuki H, Omata M.
Journal J Virol Methods
Impact factor
1.76
Citation count: 9
The second set of primer and probe sequences (CDC-N2) based on the Nucleocapsid gene developed by CDC, used in real-time RT-PCR assay. 32650037
(J Virol Methods)
PMID
32650037
Date of Publishing: 2020 Oct
Title Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay
Author(s) name Hirotsu Y, Mochizuki H, Omata M.
Journal J Virol Methods
Impact factor
1.76
Citation count: 9
The first set of primer and probe sequences (CDC-N1) based on the Nucleocapsid gene developed by CDC, used in real-time RT-PCR assay. 32650037
(J Virol Methods)
PMID
32650037
Date of Publishing: 2020 Oct
Title Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay
Author(s) name Hirotsu Y, Mochizuki H, Omata M.
Journal J Virol Methods
Impact factor
1.76
Citation count: 9
Primer and probe sequences, developed by US CDC RT-qPCR panel, specific for detecting Nucleocapsid gene of SARS-CoV-2, SARS-CoV, and other Sarbecoviruses. 32396505
(Emerg Infect Dis)
PMID
32396505
Date of Publishing: 2020 Aug
Title US CDC Real-Time Reverse Transcription PCR Panel for detection of Severe Acute Respiratory Syndrome Coronavirus 3
Author(s) nameLu X, Wang L et al.
Journal Emerg Infect Dis
Impact factor
6.81
Citation count: 38
Primer and probe sequences, developed by US CDC RT-qPCR panel, specific for detecting N2 site (Nucleocapsid gene) of SARS-CoV-2. 32396505
(Emerg Infect Dis)
PMID
32396505
Date of Publishing: 2020 Aug
Title US CDC Real-Time Reverse Transcription PCR Panel for detection of Severe Acute Respiratory Syndrome Coronavirus 3
Author(s) nameLu X, Wang L et al.
Journal Emerg Infect Dis
Impact factor
6.81
Citation count: 38
Primer and probe sequences, developed by US CDC RT-qPCR panel, specific for detecting N1 site (Nucleocapsid gene) of SARS-CoV-2. 32396505
(Emerg Infect Dis)
PMID
32396505
Date of Publishing: 2020 Aug
Title US CDC Real-Time Reverse Transcription PCR Panel for detection of Severe Acute Respiratory Syndrome Coronavirus 3
Author(s) nameLu X, Wang L et al.
Journal Emerg Infect Dis
Impact factor
6.81
Citation count: 38
Multiple sequence alignment of nucleocapsid protein of coronaviruses N-terminal domain 32363136
(Acta Pharm Sin B)
PMID
32363136
Date of Publishing: 2020 Jul
Title Crystal structure of SARS-CoV-2 nucleocapsid protein RNA binding domain reveals potential unique drug targeting sites
Author(s) nameKang S, Yang M et al.
Journal Acta Pharm Sin B
Impact factor
6.15
Citation count: 72
Primer probe sequence for Nucleocapsid protein (Set No. 2) used in real-time RT-PCR 32074516
(Jpn J Infect Dis)
PMID
32074516
Date of Publishing: 2020 Jul 22
Title Development of genetic diagnostoc methods for detection for Novel Coronavirus 2019 (nCoV-2019) in Japan
Author(s) nameShirato K, Nao N et al.
Journal Jpn J Infect Dis
Impact factor
1.11
Citation count: 72
Primer probe sequence for Nucleocapsid protein (Set No.1) used in real-time RT-PCR 32074516
(Jpn J Infect Dis)
PMID
32074516
Date of Publishing: 2020 Jul 22
Title Development of genetic diagnostoc methods for detection for Novel Coronavirus 2019 (nCoV-2019) in Japan
Author(s) nameShirato K, Nao N et al.
Journal Jpn J Infect Dis
Impact factor
1.11
Citation count: 72

Structure : Nucleocapsid

Total row(s): 2
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Key Findings
Original Article
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Structural characterization of SARS-CoV-2 nucleocapsid protein N-terminal RNA binding domain, comparison of SARS-CoV-2 N-NTD with related viral N-NTD structures and identifying a potential unique drug target pocket in SARS-CoV-2 N-NTD 32363136
(Acta Pharm Sin B)
PMID
32363136
Date of Publishing: 2020 Jul
Title Crystal structure of SARS-CoV-2 nucleocapsid protein RNA binding domain reveals potential unique drug targeting sites
Author(s) nameKang S, Yang M et al.
Journal Acta Pharm Sin B
Impact factor
6.15
Citation count: 72
Structural characterization of SARS-CoV-2 nucleocapsid protein N-terminal RNA binding domain, comparison of SARS-CoV-2 N-NTD with related viral N-NTD structures and identifying a potential unique drug target pocket in SARS-CoV-2 N-NTD
Title Crystal structure of SARS-CoV-2 nucleocapsid protein RNA binding domain reveals potential unique drug targeting sites

Drugs : Nucleocapsid

Total row(s): 4
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Key Findings
Original Article
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Different potential repurposed drugs, including hydroxychloroquine, remdesivir, Lopinavir and Affatinib..etc, i.e total 131 drugs were screened in the present study. Molecular docking of these drugs with different SARS-CoV-2 target proteins, including main protease of the virus MPro, Papain-like Protease from SARS-COV-2PLPro, N-terminal RNA binding domain of Nucleocapsid protein of SARS-COV-2 and RNA dependent RNA Polymerase from SARS-COV-2 was performed. Molecular dynamics simulation and MM-PBSA calculation were also conducted. This study showed that among tested drugs in the present in silico study, Afatinib has the highest binding potential to the main protease of SARS-CoV-2, which is higher than HCQ and remdesivir, respectively. 34032180
(J Biomol Struct Dyn)
PMID
34032180
Date of Publishing: 2021 May 25
Title Virtual screening of quinoline derived library for SARS-COV-2 targeting viral entry and replication
Author(s) nameAnju A, Chaturvedi S et al.
Journal J Biomol Struct Dyn
Impact factor
3.22
Citation count: 1
Date of Entry 2021 Sep 5
Type I PRMT inhibitor (MS023) inhibits interaction of N protein with the 5-UTR of SARS-CoV-2 genomic RNA, a property required for viral packaging. 34029587
(J Biol Chem)
PMID
34029587
Date of Publishing: 2021 May 23
Title Arginine methylation of SARS-Cov-2 nucleocapsid protein regulates RNA binding, its ability to suppress stress granule formation, and viral replication
Author(s) nameCai T, Yu Z et al.
Journal J Biol Chem
Impact factor
3.96
Citation count: 1
Date of Entry 2021 Jul 28
The study analyzed the phylogenetic relationship of N protein sequence divergence with other 49 coronavirus species and also identified the conserved regions according to protein families through conserved domain search. The analyzed compounds presented the higher numbers of hydrogen bonds of selected chemicals supporting the drug-ability of these compounds. Among them, the established antiviral drug glycyrrhizic acid and the phytochemical theaflavin can be considered as possible drug compounds against target N protein of SARS-CoV-2 as they showed lower binding affinities. 33412759
(Genomics Inform)
PMID
33412759
Date of Publishing: 2020 Dec
Title Druggability for COVID-19: in silico discovery of potential drug compounds against nucleocapsid (N) protein of SARS-CoV-2
Author(s) name Ray M, Sarkar S, Rath SN.
Journal Genomics Inform
Citation count 3
Date of Entry 2021 Sep 5
This study addresses the potential to repurpose antiviral compounds approved or in development for treating human CoV induced infections against SARSCoV2 N. The results of this analysis indicate that rapamycin, saracatinib, camostat, trametinib, and nafamostat were the top hit compounds. These results suggest that these residues are potential drug targeting sites for the SARSCoV2 N protein. 33314794
(Biotechnol Prog)
PMID
33314794
Date of Publishing: 2020 Dec 11
Title Computational drug repurposing study of the RNA binding domain of SARSCoV2 nucleocapsid protein with antiviral agents
Author(s) name Tatar G, Ozyurt E, Turhan K.
Journal Biotechnol Prog
Impact factor
2.51
Citation count: 1
Date of Entry 2021 Sep 5

Diagnostics : Nucleocapsid

Total row(s): 7
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Key Findings
Original Article
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Detection of SARS CoV-2 IgM, and IgA antibodies from Sera samples using Luminex bead-based assay by targeting SARS-CoV-2 spike and nucleocapsid antigens. The neutralizing and binding IgG, IgA, and IgM antibody levels were higher for severe than mild cases in the early convalescent phase (<6 weeks). 33227340
(J Virol Methods)
PMID
33227340
Date of Publishing: 2021 Feb
Title Evaluating SARS-CoV-2 spike and nucleocapsid proteins as targets for antibody detection in severe and mild COVID-19 cases using a Luminex bead-based assay
Author(s) nameMariƫn J, Ceulemans A et al.
Journal J Virol Methods
Impact factor
1.76
Citation count: 1
In this study, a nucleocapsid protein antigen test, based on fluorescence immunochromatographic (FIC) assay, was evaluated for the rapid detection of SARS-CoV-2 N protein antigen. With RT-PCR assay as the reference standard, this assay detected SARS-CoV-2 infection in 10 minutes, with the sensitivity, specificity, and percentage agreement of 75.6%, 100%, and 80.5%, respectively. 33031947
(Clin Microbiol Infect)
PMID
33031947
Date of Publishing: 2020 Oct 5
Title Accuracy of a nucleocapsid protein antigen rapid test in the diagnosis of SARS-CoV-2 infection
Author(s) nameDiao B, Wen K et al.
Journal Clin Microbiol Infect
Impact factor
6.17
Citation count: 9
Reporting DIOS RT-qPCR assay, which targets two regions of nucleocapsid genes (N1, N2) to detect SARS-CoV-2. 32824767
(Diagnostics (Basel))
PMID
32824767
Date of Publishing: 2020 Aug 18
Title Direct-RT-qPCR detection of SARS-CoV-2 without RNA extraction as part of a Covid-19 testing strategy: From sample to result in one hour
Author(s) name Kriegova E, Fillerova R, Kvapil P.
Journal Diagnostics (Basel)
Impact factor
2.36
Citation count: 3
A chemiluminescent assay that detects IgG antibodies against nucleocapsid gene with utmost specificity 32710669
(J Med Virol)
PMID
32710669
Date of Publishing: 2021 Feb
Title Validation and performance comparision of three SARS-CoV-2 antibody assays
Author(s) namePaiva KJ, Grisson RD et al.
Journal J Med Virol
Impact factor
2.07
Citation count: 4
Reporting diagnostic panel developed by US CDC consists of 3 real-time reverse transcription PCR assays specific for detecting the nucleocapsid gene of SARS-COV-2. 32396505
(Emerg Infect Dis)
PMID
32396505
Date of Publishing: 2020 Aug
Title US CDC Real-Time Reverse Transcription PCR Panel for detection of Severe Acute Respiratory Syndrome Coronavirus 2
Author(s) nameLu X, Wang L et al.
Journal Emerg Infect Dis
Impact factor
6.81
Citation count: 38
In this study, a clinically useful timeline of two diagnostic tests, reverse transcriptase-polymerase chain reaction and IgM and IgG enzyme-linked immunosorbent assay is reported, by using data from several published reports for detection of COVID-19. The study revealed that the nucleocapsid and RBD-S antigens showed a high sensitivity for detecting the infection. The serological assay performed two weeks later of symptoms onset had more diagnostic accuracy. 32374370
(JAMA)
PMID
32374370
Date of Publishing: 2020 Jun 9
Title Interpreting diagnostic tests for SARS CoV-2
Author(s) name Sethuraman N, Jeremiah SS, Ryo A.
Journal JAMA
Impact factor
14.78
Citation count: 209
The implementation of real-time RT-PCR for the detection of COVID-19 based on SARS-CoV-2 nucleocapsid gene set 1 and 2. 32074516
(Jpn J Infect Dis)
PMID
32074516
Date of Publishing: 2020 Jul 22
Title Development of genetic diagnostic methods for detection for Novel Coronavirus 2019 (nCoV-2019) in Japan
Author(s) nameShirato K, Nao N et al.
Journal Jpn J Infect Dis
Impact factor
1.11
Citation count: 72

Molecular_interactions : Nucleocapsid

Total row(s): 1
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Key Findings
Original Article
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Host protein arginine methyltransferases (PRMTs) methylates SARS-CoV-2 N protein at residues R95 and R177 within RGG/RG motifs.Type I PRMT inhibitor (MS023) or substitution of R95 or R177 with lysine inhibits interaction of N protein with the 5-UTR of SARS-CoV-2 genomic RNA, a property required for viral packaging. 34029587
(J Biol Chem)
PMID
34029587
Date of Publishing: 2021 May 23
Title Arginine methylation of SARS-Cov-2 nucleocapsid protein regulates RNA binding, its ability to suppress stress granule formation, and viral replication
Author(s) nameCai T, Yu Z et al.
Journal J Biol Chem
Impact factor
3.96
Citation count: 1
Date of Entry 2021 Jul 28

Immunology : Nucleocapsid

Total row(s): 14
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Key Findings
Original Article
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The SARS-CoV-2 immune memory kinetics was assessed more than 6 months after infection. Spike protein-specific memory B cells were higher in number at 6 months than one month after infection. The CD4+ and CD8+ T cells decreased with a half-life of 3-5 months. 33408181
(Science)
PMID
33408181
Date of Publishing: 2021 Jan 6
Title Immunological memory to SARS-CoV-2 assessed for up to 8 months after infection
Author(s) nameDan JM, Mateus J et al.
Journal Science
Impact factor
20.57
Citation count: 9
Date of Entry 2021 Jul 24
34 cytotoxic T cell epitopes located in spike glycoprotein, envelope protein, membrane protein, and nucleocapsid phosphoprotein were identified. 33257716
(Sci Rep)
PMID
33257716
Date of Publishing: 2020 Nov 30
Title Immunoinformatic design of a COVID-19 subunit vaccine using entire structural immunogenic epitopes of SARS-CoV-2
Author(s) nameBehmard E, Soleymani B et al.
Journal Sci Rep
Impact factor
4.12
Citation count: 1
14 helper T cell epitopes located in spike glycoprotein, envelope protein, and nucleocapsid phosphoprotein were identified. 33257716
(Sci Rep)
PMID
33257716
Date of Publishing: 2020 Nov 30
Title Immunoinformatic design of a COVID-19 subunit vaccine using entire structural immunogenic epitopes of SARS-CoV-2
Author(s) nameBehmard E, Soleymani B et al.
Journal Sci Rep
Impact factor
4.12
Citation count: 1
The T cell response against a variety of structural and non-structural proteins revealed a coordinated and dynamic immune response. A broad range of SARS-CoV-2 proteome epitopes is recognized by the CD8+ T cell responses. 33052343
(bioRxiv)
PMID
33052343
Date of Publishing: 2020 Oct 8
Title CD8+ T cell responses in convalescent COVID-19 individuals target epitopes from the entire SARS-CoV-2 proteome and show kinetics of early differentiation
Author(s) nameKared H, Redd AD et al.
Journal bioRxiv
Impact factor
Its not a journal
Citation count: 2
Four linear B-cell epitopes on the S and N viral proteins were identified. 32711254
(EBioMedicine)
PMID
32711254
Date of Publishing: 2020 Aug
Title Linear B-cell epitopes in the spike and nucleocapsid proteins as markers of SARS-CoV-2 exposure and disease severity
Author(s) nameAmrun SN, Lee CY et al.
Journal EBioMedicine
Impact factor
6.49
Citation count: 9
Chemiluminescent microparticle immunoassay (CMIA) was used to measure the IgG antibodies against SARS-CoV-2 nucleocapsid protein (NCP) and a lab-developed proteome array was used to detect IgM against NCP. There was no significant difference in the IgG antibody levels between the patients with mild/moderate SARS-CoV-2 infection and those with severe disease. 32666092
(Am J Clin Pathol)
PMID
32666092
Date of Publishing: 2020 Sep 8
Title SARS-CoV-2 Antibody Responses Do Not Predict COVID-19 Disease Severity
Author(s) namePhipps WS, SoRelle JA et al.
Journal Am J Clin Pathol
Impact factor
2.03
Citation count: 8
Date of Entry 2021 Sep 4
The kinetics of nucleocapsid and spike specific IgM and IgG responses in ICU and non-ICU COVID-19 patients were studied. 32357808
(Emerg Microbes Infect)
PMID
32357808
Date of Publishing: 2020 Dec
Title Kinetics of SARS-CoV-2 specific IgM and IgG responses in COVID-19 patients
Author(s) nameSun B, Feng Y et al.
Journal Emerg Microbes Infect
Impact factor
5.84
Citation count: 70
Seroconversion patterns of IgG and IgM antibodies(nucleocapsid (N) protein and spike (S) glycoprotein) in patients infected with SARS-CoV-2 show that the seroconversion time of the IgG antibody was earlier than that of IgM antibody. 32337590
(Clin Infect Dis)
PMID
32337590
Date of Publishing: 2020 Nov 19
Title Profile of Immunoglobulin G and IgM Antibodies Against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
Author(s) nameQu J, Wu C et al.
Journal Clin Infect Dis
Impact factor
7.71
Citation count: 56
Two (conserved) epitopes from nucleocapsid protein of SARS-CoV-2 were identified by in silico method. 32302675
(Microbes Infect)
PMID
32302675
Date of Publishing: 2020 May-Jun
Title Comparative computational analysis of SARS-CoV-2 nucleocapsid protein epitopes in taxonomically related coronaviruses
Author(s) nameTilocca B, Soggiu A et al.
Journal Microbes Infect
Impact factor
2.373
Citation count: 33
Three (conserved) epitopes from nucleocapsid protein of SARS-CoV-2 were identified by In-silico method. 32302675
(Microbes Infect)
PMID
32302675
Date of Publishing: 2020 May-Jun
Title Comparative computational analysis of SARS-CoV-2 nucleocapsid protein epitopes in taxonomically related coronaviruses
Author(s) nameTilocca B, Soggiu A et al.
Journal Microbes Infect
Impact factor
2.373
Citation count: 33
Epitopes of bat RaTG13 coronavirus and SARS-CoV showed 100% sequence identity with SARS-CoV-2 epitopes, whereas some pangolin NC protein epitopes showed a lower identity percentage. 32302675
(Microbes Infect)
PMID
32302675
Date of Publishing: 2020 May-Jun
Title Comparative computational analysis of SARS-CoV-2 nucleocapsid protein epitopes in taxonomically related coronaviruses
Author(s) nameTilocca B, Soggiu A et al.
Journal Microbes Infect
Impact factor
2.373
Citation count: 33