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Last updated: 2021 Nov 2
Total hit(s): 10
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Original Article
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The kinetics of binding of soluble ACE2 to recombinant RBD was assessed by biolayer interferometry (BLI). The affinity of the B.1.351 RBD to ACE2 was 19-fold higher when compared to the wildtype and 2.7-fold higher when compared to the alpha variant.
33730597
(Cell)
PMID
33730597
Date of Publishing: 2021 Apr 29
Title Evidence of escape of SARS-CoV-2 variant B.1.351 from natural and vaccine-induced sera
Author(s) nameZhou D, Dejnirattisai W et al.
Journal Cell
Impact factor
27.35
Citation count: 36
Date of Entry 2021 Nov 2


SARS-CoV-2 Delta variant has a higher replication fitness compared to Alpha variant as studied in in-vitro respiratory SARS-CoV-2 models. The Delta vs Alpha RNA ratio increased from 1.7 on day 1 to 3.1 on day 5. The furin cleavage site mutation P681R plays a key function in increasing the Delta variant's replication in basic human airway cultures.
Pre-print ( bioRXiv )
Title Delta spike P681R mutation enhances SARS-CoV-2 fitness over Alpha variant
Impact factor
N/A
Date of Entry 2021 Sep 30


The D614G spike mutation results in increased viral transduction. However, there was no change in the binding profiles of soluble D614 and G614 spike to human ACE2. The two dissociation constants (KD) were: KD1 = 8.45 nM and KD2 = 127 nM for D614 variant, and KD1 = 18.0 nM and KD2 = 92.7 nM for G614 variant.
32587969 (33570490)
(bioRxiv)
PMID
32587969 (33570490)
Date of Publishing: 2020 Jun 15
Title The Spike D614G mutation increases SARS-CoV-2 infection of multiple human cell types
Author(s) name Daniloski Z, Guo X, Sanjana NE.
Journal bioRxiv
Impact factor
N/A
Citation count: 2
Date of Entry 2021 Sep 13


Structure analysis of SARS-CoV-2 N439K Receptor Binding Domain (RBD) in complex with hACE2 at 2.8 resolution showed strong non-covalent salt bridges. These bridges enhanced the binding for hACE2 in the N439K variant, as similar to the SARS-CoV N439R variant. Double mutants of N439K/R and K417V mutations show that salt bridge loss at RBD position 417 is compensated by the one at position 439. This results in hACE2 affinity similar to the wild type. Methods used for study: X-ray structure, Surface plasmon resonance (SPR)
33621484
(Cell)
PMID
33621484
Date of Publishing: 2021 Jan 28
Title Circulating SARS-CoV-2 spike N439K variants maintain fitness while evading antibody-mediated immunity
Author(s) nameThomson EC, Rosen LE et al.
Journal Cell
Impact factor
27.35
Citation count: 1
Date of Entry 2021 Aug 6


The binding kinetics of the spike protein of alpha variant and spike of the D614G variant was compared by biolayer interferometry (BLI). A 2-fold decrease was observed in the dissociation constant (Kd) of spike in the alpha variant (Kd=1.3nM) compared to the D614G variant (Kd=3.1nM).
Pre-print ( bioRXiv )
Title Impacts on the structure-function relationship of SARS-CoV-2 spike by B.1.1.7 mutations
Impact factor
N/A
Date of Entry 2021 Aug 6


The Y453F and L452R mutations significantly increased the binding affinity to human ACE2 (RBD parental KD = 2.05 0.26 nM; RBD Y453F KD = 0.51 0.06 nM; RBD L452R KD = 1.20 0.06 nM)
Pre-print ( bioRXiv )
Title An emerging SARS-CoV-2 mutant evading cellular immunity and increasing viral infectivity
Impact factor
N/A
Date of Entry 2021 Aug 6


There were no changes in the symptoms or duration of the disease in cases associated with the SARS-CoV-2 Alpha variant. Vaccines were likely to remain effective against the Alpha variant owing to no changes in the reinfection rates.
doi
Date of Publishing 2021 Apr 12
Title Changes in symptomatology, reinfection, and transmissibility associated with the SARS-CoV-2 variant B.1.1.7: an ecological study
Impact factor
N/A
Date of Entry 2021 Jul 2


The most common symptoms associated with the SARS-CoV-2 Alpha variant was Sore throat, Myalgia, and Fatigue.
doi
Date of Publishing 2021 Jan 27
Title Covid-19: Sore throat, fatigue, and myalgia are more common with new UK variant
Impact factor
N/A
Date of Entry 2021 Jul 2


A multiplex RT-qPCR assay was designed to identify and differentiate between the B.1.1.7 and the B.1.351 variants. The assay can detect the B.1.1.7 specific D3L mutation and the B.1.351 specific 242-244 deletion mutation. This assay can be used for the rapid and precise differentiation of the alpha and beta variants
Pre-print ( medRXiv )
Date of Publishing 2021 May 22
Title Rapid And high throughput RT-qPCR assay for identification and differentiation between SARS-CoV-2 variants-Alpha (B.1.1.7) and Beta (B.1.351)
Impact factor
N/A
Date of Entry 2021 Jul


Xenonucleic acids (XNA)-based multiplex qPCR testing kit was developed to detect the SARS CoV-2 variants. The kit helps in identifying the D614G mutation and N501Y mutations in SARS-CoV-2. Owing to the stronger hybridization of XNA/DNA than DNA, XNAs are used to target specific nucleic sequences allowing for more accurate and robust results. DG614 variant (B.1.1.7) is now prevalent in the United States due to its higher transmissibility and infective rates than expected. N501Y variant belonging to the clade of the former has independently developed in the UK and has the potential to emerge as the dominant variant.
Pre-print ( medRXiv )
Date of Publishing 2021 Apr 05
Title A Rapid SARS-CoV-2 Variant Detection by Molecular-Clamping Based RT-qPCR
Impact factor
N/A
Date of Entry 2021 Jul