Primers and Probes


Last updated: 2021 Jun 14
Total hit(s): 64
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Original Article
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Primer and Probe sequence specific for N gene of SARS-CoV-2 used in wastewater RT- ddPCR assay. Amplicon Length: 95
Genomic Location of forward primer: 20131-29150,
Genomic Location of reverse primer: 29225-29206,
Genomic location of probe sequence: 29185-29204
Pre-print (medRXiv)
Date of Publishing -
Title Redesigning SARS-CoV-2 clinical RT-qPCR assays for wastewater RT-ddPCR
Author(s) name -
Date of Entry 2021 Jun 14


Primer and Probe sequence specific for E gene of SARS-CoV-2 used in wastewater RT- ddPCR assay. Amplicon Length: 100
Genomic Location of forward primer: 26260-26279,
Genomic Location of reverse primer: 26359-26340,
Genomic location of probe sequence: 26290-26317
Pre-print (medRXiv)
Date of Publishing -
Title Redesigning SARS-CoV-2 clinical RT-qPCR assays for wastewater RT-ddPCR
Author(s) name -
Date of Entry 2021 Jun 14


Outer primer pairs for an RT-LAMP Cas12a assay specific for the N gene of SARS-CoV-2.
33238709
(Anal Chem)
PMID
33238709
Date of Publishing: 2020 Dec 15
Title Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology
Author(s) namePang B, Xu J et al.
Journal Anal Chem
Impact factor
6.37
Citation count: 1


Inner primer pairs (FIP, BIP) for an RT-LAMP Cas12a assay specific for the N gene of SARS-CoV-2.
33238709
(Anal Chem)
PMID
33238709
Date of Publishing: 2020 Dec 15
Title Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology
Author(s) namePang B, Xu J et al.
Journal Anal Chem
Impact factor
6.37
Citation count: 1


Loop primer pairs (LF and LB) for an RT-LAMP Cas12a assay specific for the N gene of SARS-CoV-2.
33238709
(Anal Chem)
PMID
33238709
Date of Publishing: 2020 Dec 15
Title Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology
Author(s) namePang B, Xu J et al.
Journal Anal Chem
Impact factor
6.37
Citation count: 1


Outer primer pairs for an RT-LAMP Cas12a assay specific for the E gene of SARS-CoV-2.
33238709
(Anal Chem)
PMID
33238709
Date of Publishing: 2020 Dec 15
Title Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology
Author(s) namePang B, Xu J et al.
Journal Anal Chem
Impact factor
6.37
Citation count: 1


Inner primer pairs (FIP, BIP) for an RT-LAMP Cas12a assay specific for the E gene of SARS-CoV-2
33238709
(Anal Chem)
PMID
33238709
Date of Publishing: 2020 Dec 15
Title Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology
Author(s) namePang B, Xu J et al.
Journal Anal Chem
Impact factor
6.37
Citation count: 1


Loop primer pairs (LF and LB) for an RT-LAMP Cas12a assay specific for the E gene of SARS-CoV-2.
33238709
(Anal Chem)
PMID
33238709
Date of Publishing: 2020 Dec 15
Title Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology
Author(s) namePang B, Xu J et al.
Journal Anal Chem
Impact factor
6.37
Citation count: 1


Reporter labeled with fluorophore and quencher specific for the purpose of fluorescence detection carried out by Cas12a-mediated RT-LAMP assay.
33238709
(Anal Chem)
PMID
33238709
Date of Publishing: 2020 Dec 15
Title Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology
Author(s) namePang B, Xu J et al.
Journal Anal Chem
Impact factor
6.37
Citation count: 1


Sequences of the DNA probes used in the HC-FIA assay specific for binding with the N region of the SARS-CoV-2 genome. The software Primer Premier 5.0, was used for designing the primer
33273714
(Nat Biomed Eng)
PMID
33273714
Date of Publishing: 2020 Dec
Title Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA
Author(s) nameWang D, He S et al.
Journal Nat Biomed Eng
Citation count 3


Sequences of the DNA probes used in the HC-FIA assay specific for binding with the ORF1ab region of the SARS-CoV-2 genome. The software Primer Premier 5.0, was used for designing the primer
33273714
(Nat Biomed Eng)
PMID
33273714
Date of Publishing: 2020 Dec
Title Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA
Author(s) nameWang D, He S et al.
Journal Nat Biomed Eng
Citation count 3


The eight specific primer sequences used in RT-LAMP assay for the detection of SARS-CoV-2.
33098286
(Am J Trop Med Hyg)
PMID
33098286
Date of Publishing: 2020 Dec
Title A sensitive reverse transcription loop mediated isothermal amplification assay for direct visual detection of SARS-CoV-2
Author(s) nameLau YL, Ismail IB et al.
Journal Am J Trop Med Hyg
Impact factor
2.25
Citation count: 1


Sequences of the DNA probes used in the HC-FIA assay specific for binding with the E region of the SARS-CoV-2 genome. The software Primer Premier 5.0, was used for designing the primer
33273714
(Nat Biomed Eng)
PMID
33273714
Date of Publishing: 2020 Dec
Title Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA
Author(s) nameWang D, He S et al.
Journal Nat Biomed Eng
Citation count 3


Primers sequence specific for the RdRp gene used in RT-LAMP assay to detect SARS-CoV-2. Primers were designed by using PrimerExplorer version 5 (http://primerexplorer.jp/e/)
33165486
(Analyst)
PMID
33165486
Date of Publishing: 2020 Nov 9
Title Colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a visual diagnostic platform for the detection of the emerging coronavirus SARS-CoV-2
Author(s) nameNawattanapaiboon K , Pasomsub E et al.
Journal Analyst
Impact factor
3.98
Citation count: 1


Probe for a real-time RT-PCR test specific for the nsp1 gene of SARS-CoV-2.
32501535
(J Med Virol)
PMID
32501535
Date of Publishing: 2020 Nov
Title Identification of nsp1 gene as the target of SARS-CoV-2 real time RT-PCR using nanopore whole genome sequencing
Author(s) nameChan WM, Ip JD et al.
Journal J Med Virol
Impact factor
2.07
Citation count: 5


Primer pairs for a real-time RT-PCR test specific for the nsp1 gene of SARS-CoV-2.
32501535
(J Med Virol)
PMID
32501535
Date of Publishing: 2020 Nov
Title Identification of nsp1 gene as the target of SARS-CoV-2 real time RT-PCR using nanopore whole genome sequencing
Author(s) nameChan WM, Ip JD et al.
Journal J Med Virol
Impact factor
2.07
Citation count: 5


Primer pairs, for a real-time RT-RAA assay, specific for the N gene of SARS CoV2.
32758712
(Virology)
PMID
32758712
Date of Publishing: 2020 Oct
Title A reverse-transcription recombinase-aided amplification assay for the rapid detection of N gene of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)
Author(s) nameWu T, Ge Y et al.
Journal Virology
Impact factor
2.819
Citation count: 4


The second set of primer and probe sequences (YCH-N2) based on the Nucleocapsid gene developed by YCH (Yamanashi Central Hospital), used in real-time RT-PCR assay. The assay uses double-quencher probe
32650037
(J Virol Methods)
PMID
32650037
Date of Publishing: 2020 Oct
Title Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay
Author(s) name Hirotsu Y, Mochizuki H, Omata M.
Journal J Virol Methods
Impact factor
1.76
Citation count: 9


The first set of primer and probe sequences (YCH-N1) based on the Nucleocapsid gene developed by YCH (Yamanashi Central Hospital), used in real-time RT-PCR assay. The expected amplicon size is 158 bp. The assay uses double-quencher probe
32650037
(J Virol Methods)
PMID
32650037
Date of Publishing: 2020 Oct
Title Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay
Author(s) name Hirotsu Y, Mochizuki H, Omata M.
Journal J Virol Methods
Impact factor
1.76
Citation count: 9


The second set of primer and probe sequences (NIID-N2) based on the Nucleocapsid gene developed by NIID, used in real-time RT-PCR assay. The assay uses single-quencher probe
32650037
(J Virol Methods)
PMID
32650037
Date of Publishing: 2020 Oct
Title Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay
Author(s) name Hirotsu Y, Mochizuki H, Omata M.
Journal J Virol Methods
Impact factor
1.76
Citation count: 9


The first set of primer and probe sequences (NIID-N1) based on the Nucleocapsid gene developed by NIID, used in real-time RT-PCR assay. The expected amplicon size is 128 bp. The assay uses single-quencher probe
32650037
(J Virol Methods)
PMID
32650037
Date of Publishing: 2020 Oct
Title Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay
Author(s) name Hirotsu Y, Mochizuki H, Omata M.
Journal J Virol Methods
Impact factor
1.76
Citation count: 9


The second set of primer and probe sequences (CDC-N2) based on the Nucleocapsid gene developed by CDC, used in real-time RT-PCR assay. The expected amplicon size is 67 bp. The assay uses single-quencher probe
32650037
(J Virol Methods)
PMID
32650037
Date of Publishing: 2020 Oct
Title Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay
Author(s) name Hirotsu Y, Mochizuki H, Omata M.
Journal J Virol Methods
Impact factor
1.76
Citation count: 9


Probe for a real-time RT-RAA assay specific for the N gene of SARS CoV2. Probe Format Modifications: FAM: 6-Carboxyfluorescein; THF: tetrahydrofuran; BHQ: black hole quencher; C3 spacer: 3 phosphate blocker.
32758712
(Virology)
PMID
32758712
Date of Publishing: 2020 Oct
Title A reverse-transcription recombinase-aided amplification assay for the rapid detection of N gene of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)
Author(s) nameWu T, Ge Y et al.
Journal Virology
Impact factor
2.819
Citation count: 4


The first set of primer and probe sequences (CDC-N1) based on the Nucleocapsid gene developed by CDC, used in real-time RT-PCR assay. The expected amplicon size is 72 bp. The assay uses single-quencher probe
32650037
(J Virol Methods)
PMID
32650037
Date of Publishing: 2020 Oct
Title Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay
Author(s) name Hirotsu Y, Mochizuki H, Omata M.
Journal J Virol Methods
Impact factor
1.76
Citation count: 9


The primer and probe sequences of RNAse P (internal positive control) developed by CDC, used in real-time RT-PCR assay. The assay uses single-quencher probe
32650037
(J Virol Methods)
PMID
32650037
Date of Publishing: 2020 Oct
Title Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay
Author(s) name Hirotsu Y, Mochizuki H, Omata M.
Journal J Virol Methods
Impact factor
1.76
Citation count: 9