Animal model


Last updated: 2021 Jun 15
Total hit(s): 15
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Original Article
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An acute lung injury model by intratracheally instilling the S1 subunit of SARS-CoV-2 Spike S protein (400 g/kg, 2 ml/kg body weight) in K18-hACE2 transgenic mice that overexpress the human receptor for SARS-CoV-2 Spike protein S, ACE2, and investigated outcomes 72 hours later. A single exposure of K18-hACE2 mice to SARS-CoV-2 Spike Protein S subunit S1 may represent a valid model of COVID-19, allow the study of the molecular mechanisms of SARS-CoV-2 induced lung injury and be useful in the investigation of potential new therapeutic approaches to the management of COVID-19 as well as future coronavirus-dependent respiratory diseases.
Direct author entry
(pre-publication)
Title Single intratracheal exposure to SARS-CoV-2 S1 spike protein induces acute lung injury in K18-hACE2 transgenic mice
Author(s) namePavel Solopov, Ruben Colunga Biancatelli et al.
Affiliations Pavel Solopov (Old Dominion University)| Ruben Colunga Biancatelli (Old Dominion University)| Elizabeth Sharlow (University of Virginia)| John Lazo (University of Virginia)| John Catravas (Old Dominion University, Old Dominion University)
Date of Entry 2021 Jun 15


4 Hamsters infected with WT SARS-CoV-2 viruses (Wuhan-1) showed extensive alveolar wall destruction, alveolar space haemorrhage, and mononuclear cell infiltration in the lungs and bodyweight loss. WT virus has led to disease severity in the hamster animal model
32301390
(Emerg Microbes Infect)
PMID
32301390
Date of Publishing: 2020 Dec
Title Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction
Author(s) nameLau SY, Wang P et al.
Journal Emerg Microbes Infect
Impact factor
5.84
Citation count: 36


4 Hamsters infected with Del-mut-1 SARS-CoV-2 viruses showed diffuse alveolar wall thickening and peribronchiolar infiltration in the lungs and no bodyweight loss. Del-mut-1 (a 30 bp deletion at S1/S2 junction) attenuated variant has reduced its ability to cause disease in the hamster animal model
32301390
(Emerg Microbes Infect)
PMID
32301390
Date of Publishing: 2020 Dec
Title Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction
Author(s) nameLau SY, Wang P et al.
Journal Emerg Microbes Infect
Impact factor
5.84
Citation count: 36


The virus titers in the tracheal and lung tissues of infected Hamsters showed higher virus replication in the lung tissues of hamsters infected with WT SARS-CoV-2 than Del-mut-1 variant infected hamsters.
32301390
(Emerg Microbes Infect)
PMID
32301390
Date of Publishing: 2020 Dec
Title Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction
Author(s) nameLau SY, Wang P et al.
Journal Emerg Microbes Infect
Impact factor
5.84
Citation count: 36


Hamsters infected with mutant SARS-CoV-2 (G614 virus) showed higher viral load in the nasal washes and the tracheae, but not in the lungs, than those of Hamsters infected with WT SARS-CoV-2 (D614 virus). Infected hamsters from both groups showed similar weight loss.
33106671
(Nature)
PMID
33106671
Date of Publishing: 2020 Oct 26
Title Spike mutation D614G alters SARS-CoV-2 fitness
Author(s) namePlante JA, Liu Y et al.
Journal Nature
Impact factor
24.36
Citation count: 38


Transgenic type I interferon knockout (IFNAR/-) mice, inoculated intranasally with SARS-CoV-2 showed infections in tissues including lungs, heart, stomach, intestines, liver, spleen and kidneys. Lungs with hyperemia, regional consolidation and subpleural air accumulation was observed on necropsy.
33015402
(Heliyon)
PMID
33015402
Date of Publishing: 2020 Sep
Title Characterization of local SARS-CoV-2 isolates and pathogenicity in IFNAR-/-mice
Author(s) nameHanifehnezhad A, Kehribar EŞ et al.
Journal Heliyon
Impact factor
1.65
Citation count: 3


2 young cynomolgus macaques infected with SARS-CoV-2 had 0-10% of lungs affected, hyaline membranes, alveolar oedema and leukocyte infiltration with type I & II pneumocytes affected at 4 days post-infection. Organs affected were also noticed for SARS-CoV and MERS-CoV.
32303590
(Science)
PMID
32303590
Date of Publishing: 2020 May 29
Title Comparative pathogenesis of COVID-19, MERS, and SARS in a nonhuman primate model
Author(s) nameRockx B, Kuiken T et al.
Journal Science
Impact factor
20.57
Citation count: 197


2 aged cynomolgus macaques infected with SARS-CoV-2 had 0-5% of lungs affected, and leukocyte infiltration with type I & II pneumocytes affected at 4 days post-infection. Organs affected were also noticed for SARS-CoV and MERS-CoV.
32303590
(Science)
PMID
32303590
Date of Publishing: 2020 May 29
Title Comparative pathogenesis of COVID-19, MERS, and SARS in a nonhuman primate model
Author(s) nameRockx B, Kuiken T et al.
Journal Science
Impact factor
20.57
Citation count: 197


Nasal turbinate, trachea, lungs, kidney, and intestine with acute bronchiolitis was present in infected lungs of ferrets intranasally infected with SARS-CoV-2 virus. After 12 dpi, viral RNA and virus recovery failed to be detected in all tested tissues.
32259477
(Cell Host Microbe)
PMID
32259477
Date of Publishing: 2020 May 13
Title Infection and Rapid Transmission of SARS-CoV-2 in Ferrets
Author(s) nameKim YI, Kim SG et al.
Journal Cell Host Microbe
Impact factor
10.45
Citation count: 181


12-24 months old male and female ferrets inoculated with SARS-CoV-2 virus had elevated body temperatures and reduced activity.
32259477
(Cell Host Microbe)
PMID
32259477
Date of Publishing: 2020 May 13
Title Infection and Rapid Transmission of SARS-CoV-2 in Ferrets
Author(s) nameKim YI, Kim SG et al.
Journal Cell Host Microbe
Impact factor
10.45
Citation count: 181


12-24 months old male and female ferrets infected through direct contact with infected ferret had elevated body temperatures and reduced activity.
32259477
(Cell Host Microbe)
PMID
32259477
Date of Publishing: 2020 May 13
Title Infection and Rapid Transmission of SARS-CoV-2 in Ferrets
Author(s) nameKim YI, Kim SG et al.
Journal Cell Host Microbe
Impact factor
10.45
Citation count: 181


12-24 months old male and female ferrets in indirect contact with infected ferrets had no change in body temperature and body weight.
32259477
(Cell Host Microbe)
PMID
32259477
Date of Publishing: 2020 May 13
Title Infection and Rapid Transmission of SARS-CoV-2 in Ferrets
Author(s) nameKim YI, Kim SG et al.
Journal Cell Host Microbe
Impact factor
10.45
Citation count: 181


12-24 months old male and female ferrets inoculated with SARS-CoV-2 virus had highest viral RNA in nasal washes. Viral RNA was also detected in saliva specimens.
32259477
(Cell Host Microbe)
PMID
32259477
Date of Publishing: 2020 May 13
Title Infection and Rapid Transmission of SARS-CoV-2 in Ferrets
Author(s) nameKim YI, Kim SG et al.
Journal Cell Host Microbe
Impact factor
10.45
Citation count: 181


12-24 months old male and female ferrets infected through direct contact with infected ferrets had viral RNA in nasal washes, saliva, fecal and urine samples.
32259477
(Cell Host Microbe)
PMID
32259477
Date of Publishing: 2020 May 13
Title Infection and Rapid Transmission of SARS-CoV-2 in Ferrets
Author(s) nameKim YI, Kim SG et al.
Journal Cell Host Microbe
Impact factor
10.45
Citation count: 181


2 out of 6 12-24 months old male and female ferrets in indirect contact with infected ferrets had viral RNA in nasal washes and fecal specimens.
32259477
(Cell Host Microbe)
PMID
32259477
Date of Publishing: 2020 May 13
Title Infection and Rapid Transmission of SARS-CoV-2 in Ferrets
Author(s) nameKim YI, Kim SG et al.
Journal Cell Host Microbe
Impact factor
10.45
Citation count: 181