RT-qPCR


Last updated: 18_40_00 Jul
Total hit(s): 2
Select item(s)
Key Findings
Comments
(You can add your comments too!)
Original Article
(hover to see details)
A multiplex RT-qPCR assay was designed to identify and differentiate between the B.1.1.7 and the B.1.351 variants. The assay can detect the B.1.1.7 specific D3L mutation and the B.1.351 specific 242-244 deletion mutation. This assay can be used for the rapid and precise differentiation of the alpha and beta variants
-
()
PMID
-
Date of Publishing: 2021 May 22
Title Rapid And high throughput RT-qPCR assay for identification and differentiation between SARS-CoV-2 variants-Alpha (B.1.1.7) and Beta (B.1.351)
Date of Entry 18_40_00 Jul


Xenonucleic acids (XNA)-based multiplex qPCR testing kit was developed to detect the SARS CoV-2 variants. The kit helps in identifying the D614G mutation and N501Y mutations in SARS-CoV-2. Owing to the stronger hybridization of XNA/DNA than DNA, XNAs are used to target specific nucleic sequences allowing for more accurate and robust results. DG614 variant (B.1.1.7) is now prevalent in the United States due to its higher transmissibility and infective rates than expected. N501Y variant belonging to the clade of the former has independently developed in the UK and has the potential to emerge as the dominant variant.
-
()
PMID
-
Date of Publishing: 2021 Apr 05
Title A Rapid SARS-CoV-2 Variant Detection by Molecular-Clamping Based RT-qPCR
Date of Entry 18_40_00 Jul