Single-tube nested (STN) RT-qPCR

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Reporting a new single-tube nested real-time RT-PCR (STN RT-PCR) assay for the diagnosis of COVID-19 based on SARS-CoV-2 RdRp/Hel and N genes. This method was found highly sensitive and specific for SARS-CoV-2 detection. STN COVID-19-N assays is more sensitive than the non-nestedNassay.
(Int J Mol Sci)
Date of Publishing: 2020 Aug 7
Title Development and Evaluation of Novel and Highly Sensitive Single-Tube Nested Real-Time RT-PCR Assays for SARS-CoV-2 Detection
Author(s) nameYip CC, Sridhar S et al.
Journal Int J Mol Sci
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Citation count: 2