RT-RAA assay

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Reporting a real-time reverse-transcription recombinase-aided amplification assay (RT-RAA) specific for the N gene of SARS-CoV-2. The analytical specificity of the real-time RT-RAA assay was determined by the samples positive for other pathogens ( influenza A, influenza B, HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU1, respiratory syncytial virus, parainfluenza virus, rhinovirus, and human metapneumovirus), and the results were negative for all the samples. The kappa value of this assay was 0.959, representing no significant difference between both the assays.
Date of Publishing: 2020 Oct
Title A reverse-transcription recombinase-aided amplification assay for the rapid detection of N gene of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Author(s) nameWu T, Ge Y et al.
Journal Virology
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Citation count: 4