RT-PCR assay


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Original Article
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Reporting a conventional one-step RT-PCR assay, specific for the E gene of SARS-CoV-2, which can be used as a lower-cost alternative method for the detection of SARS-CoV-2 in remote places. The conventional PCR detected until 1e-7 dilutions of the isolates. The highest Ct detected by conventional PCR was 35.98
32767275
(Braz J Microbiol)
PMID
32767275
Date of Publishing: 2020 Sep
Title Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR
Author(s) nameDorlass EG, Monteiro CO et al.
Journal Braz J Microbiol
Impact factor
1.67
Citation count: 2


Reporting a conventional two-step RT-PCR assay, specific for the E gene of SARS-CoV-2, which can be used as a lower-cost alternative method for the detection of SARS-CoV-2 in remote places. The conventional PCR detected until 1e-7 dilutions of the isolates. The highest Ct detected by conventional PCR was 35.98
32767275
(Braz J Microbiol)
PMID
32767275
Date of Publishing: 2020 Sep
Title Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR
Author(s) nameDorlass EG, Monteiro CO et al.
Journal Braz J Microbiol
Impact factor
1.67
Citation count: 2


Reporting one step nested RT-PCR, specific for ORF1ab gene, which performs two successive rounds of amplification at low cost with greater flexibility. Assay efficiency was found to be 99.66%. 183 bp sequence of ORF1ab gene (region 3801 to 3983 bp) of the reference genome (accession number: MN908947.3) has 100% similarity to all SARS-CoV-2 sequences reported in the GenBank, and 86.96% similarity with the bat SARS-like coronavirus isolate sequence. The 1COFw and 1CORw primers can be used separately for COVID-19 diagnosis from clinical samples.
32794453
(J Infect Dev Ctries)
PMID
32794453
Date of Publishing: 2020 Jul 31
Title One-step nested RT-PCR for COVID-19 detection: A flexible, locally developed test for SARS-CoV2 nucleic acid detection
Author(s) nameMeza-Robles C, Barajas-Saucedo CE et al.
Journal J Infect Dev Ctries
Impact factor
1.26
Citation count: 4


The implementation of Nested RT-PCR for the detection of COVID-19, based on SARS-CoV-2 Orf1a and S genes.
32074516
(Jpn J Infect Dis)
PMID
32074516
Date of Publishing: 2020 Jul 22
Title Development of genetic diagnostic methods for detection for Novel Coronavirus 2019 (nCoV-2019) in Japan
Author(s) nameShirato K, Nao N et al.
Journal Jpn J Infect Dis
Impact factor
1.11
Citation count: 72


In this study, a clinically useful timeline of two diagnostic tests, reverse transcriptase-polymerase chain reaction and IgM and IgG enzyme-linked immunosorbent assay is reported, by using data from several published reports for detection of COVID-19. For symptomatic patients, viral RNA in the nasopharyngeal swab becomes detectable as early as day 1 of symptoms onset, peaks within the first week, declines by the third week, and subsequently becomes undetectable. In the study, most data are for adult populations who are not immunocompromised.
32374370
(JAMA)
PMID
32374370
Date of Publishing: 2020 Jun 9
Title Interpreting diagnostic tests for SARS CoV-2
Author(s) name Sethuraman N, Jeremiah SS, Ryo A.
Journal JAMA
Impact factor
14.78
Citation count: 209