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Last updated: 2021 Jun 14
Total hit(s): 30
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In this research study, N- and E- gene multiplex (ddCoV_N and ddCoV_E) RT-ddPCR method is redesigned and optimized to detect SARS-CoV-2 from wastewater samples and compared with the standard nCoV_N2 and E_Sarbeco RT-qPCR assays. SARS-CoV-2 wastewater surveillance gives the community-level infection trends and screens infected individuals at the building level.
Differences within gene assays (ddCoV_N, ddCoV_E, and nCoV_N2, E_Sarbeco) were small because the primer and probe sequences were designed around the same genomic locations.
Pre-print (medRXiv)
Date of Publishing -
Title Redesigning SARS-CoV-2 clinical RT-qPCR assays for wastewater RT-ddPCR
Author(s) name -
Date of Entry 2021 Jun 14


This study reports a rapid and inexpensive diagnostic test, called RAPID 1.0 (Real-time Accurate Portable Impedimetric Detection prototype 1.0) which provides a result within 4 minutes. The RAPID technology is based on electrochemical impedance spectroscopy (EIS), which transforms the binding event between the SARS-CoV-2 spike protein and receptor protein ACE2 into a detected electrical signal. The signal can be read through a desktop instrument or a smartphone. RAPID 1.0 is a good alternative to sophisticated diagnostic technique like RT-PCR. RAPID is inexpensive compared to existing methods for SARS-CoV-2 detection
33997767
(Matter)
PMID
33997767
Date of Publishing: 2021 Jul 7
Title Low-cost Biosensor for Rapid Detection of SARS-CoV-2 at the Point-of-Care
Author(s) nameTorres MDT, de Araujo WR et al.
Journal Matter


Reporting an AI-based diagnosis method that integrates Self-supervised Learning as a plugin module into a Multi-task Convolutional Neural Network for the automatic diagnosis of COVID-19 based on both CT and X-ray datasets. The three CNNs applied to diagnose COVID-19 under single task schemes are : (i) VGG-19 (ii) ResNet-50 (iii) EfficientNet
33518812
(Pattern Recognit)
PMID
33518812
Date of Publishing: 2021 Jun
Title Multi-task contrastive learning for automatic CT and X-ray diagnosis of COVID-19
Author(s) nameLi J, Zhao G et al.
Journal Pattern Recognit
Impact factor
7.35
Citation count: 1


Reporting one-step RT-dPCR (reverse transcription digital PCR ) method based on SARS-CoV-2 ORF1ab, N, and E gene for detecting SARS-CoV-2 from pharyngeal swab samples. RT dPCR uses droplets of samples with as low as 2 gene copies to diagnose COVID-19 positive. The Specificity of the assays were determined by testing Influenza virus and other human coronavirus and tests gave negative results.
33379001
(Talanta)
PMID
33379001
Date of Publishing: 2021 Mar 1
Title Highly accurate and sensitive diagnostic detection of SARS-CoV-2 by digital PCR
Author(s) nameDong L, Zhou J et al.
Journal Talanta
Citation count 2


Immunosensor based quantification of the ultralow concentration of cytokines (IL-6, TNF-alpha, etc.) present in blood or respiratory samples to monitor local and systemic inflammation. Limitation : Biosensors may have a drawback when detecting IL-6 in real matrices as proteins and mucin concentrations may interfere.
33519090
(Sens Actuators B Chem)
PMID
33519090
Date of Publishing: 2021 Mar 1
Title Paper biosensors for detecting elevated IL-6 levels in blood and respiratory samples from COVID-19 patients
Author(s) nameAdrover-Jaume C, Alba-Patiño A et al.
Journal Sens Actuators B Chem
Citation count 1


A new nanotechnology-based PCR-assisted colorimetric SARS-CoV-2 detection assay works on the principle of 5'-exonuclease activity of polymerase and linker-based single-component assembly of gold nanoparticle-core spherical nucleic acids. The positive and negative viral samples produce red and purple colors in the post-PCR colorimetric test, respectively.
33012989
(Sens Actuators B Chem)
PMID
33012989
Date of Publishing: 2021 Feb 1
Title Conventional PCR Assisted Single-Component Assembly of Spherical Nucleic Acids for Simple Colorimetric Detection of SARS-CoV-2
Author(s) nameKarami A, Hasani M et al.
Journal Sens Actuators B Chem
Citation count 2


Compared to the SARS-CoV-2 RT-PCR assay, the SARS-CoV-2 Rapid Antigen Test (Roche, Switzerland) showed less sensitivity and specificity. The Rapid Antigen test provided an advantage of quick detection of infected people.
33227341
(J Virol Methods)
PMID
33227341
Date of Publishing: 2021 Feb
Title Comparision of the SARS-CoV-2 rapid antigen test to real star SARS-CoV-2 RT-PCR kit
Author(s) nameKrüttgen A, Cornelissen CG et al.
Journal J Virol Methods
Impact factor
1.76
Citation count: 4


In this study, the Rapid Antigen detection kit, Roche SARS-CoV-2 Rapid Antigen Test (RAT), a chromatographic immunoassay, was evaluated for the rapid detection of SARS-CoV-2 N protein in PCR-positive and PCR-negative swab samples. The kit produced false-negative results, with overall clinical sensitivity and specificity of 50.3% and 97.7%, respectively. RAT test was extensively flexible with the way samples were stored and used for testing.
The use of oropharyngeal sample as an alternative is not beneficial for RAT test.
33452927
(Med Microbiol Immunol)
PMID
33452927
Date of Publishing: 2021 Jan 16
Title Evaluation of two rapid antigen tests to detect SARS-CoV-2 in a hospital setting
Author(s) nameOsterman A, Baldauf HM et al.
Journal Med Microbiol Immunol
Citation count 1


In this study, the Rapid Antigen detection kit, STANDARD F COVID-19 Ag FIA (FIA), a fluorescent immunoassay, was evaluated for the rapid detection of SARS-CoV-2 nucleoprotein in PCR-positive and PCR-negative swab samples. The kit produced false-negative results, with overall clinical sensitivity and specificity of 45.4% and 97.8%, respectively. Prior storage of the specimen at -20°C slightly enhances the rate of SARS-CoV-2 antigen test.
Also the oropharyngeal can be used as an alternative sampling site for FIA.
33452927
(Med Microbiol Immunol)
PMID
33452927
Date of Publishing: 2021 Jan 16
Title Evaluation of two rapid antigen tests to detect SARS-CoV-2 in a hospital setting
Author(s) nameOsterman A, Baldauf HM et al.
Journal Med Microbiol Immunol
Citation count 1


Reporting an amplification-free, fluorescence-based diagnostic method, namely Hybrid Capture Fluorescence Immunoassay (HC-FIA), for diagnosing SARS-CoV-2. It is carried on a lateral flow strip and targets ORF1ab, E, and the N regions of the genome to detect SARS-CoV-2. The assay resulted in 100% and 99% sensitivities and specificities, respectively. This assay is based on the capture of RNA-DNA hybrids and on immunofluorescence analysis. The SARS-CoV-2 HC-FIA detection system Fig.1 (https://www.nature.com/articles/s41551-020-00655-z#Fig1)
33273714
(Nat Biomed Eng)
PMID
33273714
Date of Publishing: 2020 Dec
Title Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA
Author(s) nameWang D, He S et al.
Journal Nat Biomed Eng
Citation count 3


Reporting a highly sensitive and specific one-pot fluorescence-based detection assay, SENSR assay (sensitive splint-based one-pot isothermal RNA ) based on RdRp gene to detect SARS-CoV-2. Clinical samples were pre-treated by either thermal (heated to 95degree Celsius for 5min) or chemical lysis (with Nonidet P-40 for 5 min) and mixed directly with the SENSR mixture.
32948855
(Nat Biomed Eng)
PMID
32948855
Date of Publishing: 2020 Dec
Title Sensitive fluorescence detection of SARS-CoV-2 RNA in clinical samples via one-pot isothermal ligation and transcription
Author(s) nameWoo CH, Jang S et al.
Journal Nat Biomed Eng
Citation count 1


Reporting a new, fast detection method, NanoPCR assay specific for the N1 and N2 gene of SARS-CoV-2 based on plasmonic RT-PCR with in-situ fluorescence signal detection. Pearson coefficient (r) values indicates strong positive correlation between two variables.
33273713
(Nat Biomed Eng)
PMID
33273713
Date of Publishing: 2020 Dec
Title Fast detection of SARS CoV-2 RNA via the integration of plasmonic thermocycling and fluoroscence detection in a portable device
Author(s) nameCheong J, Yu H et al.
Journal Nat Biomed Eng
Citation count 2


An alternative method for the detection of SARS-CoV-2 directly from clinical specimens by application of direct Oxford Nanopore long-read third-generation metatranscriptomic and metagenomic sequencing technology. mNGS provided valuable information about the patients' respiratory microbiome and its relationship to coinfections. No SARS-CoV-2 reads were aligned to any of the ten samples that were suspected of having SARS-CoV-2 infection but were negative by RT-PCR
33219095
(mBio)
PMID
33219095
Date of Publishing: 2020 Nov 20
Title Metagenomic Next-Generation Sequencing of Nasopharyngeal Specimens Collected from Confirmed and Suspect COVID-19 Patients
Author(s) nameMostafa HH, Fissel JA et al.
Journal mBio
Impact factor
6.5
Citation count: 2


In this study, a nucleocapsid protein antigen test, based on fluorescence immunochromatographic (FIC) assay, was evaluated for the rapid detection of SARS-CoV-2 N protein antigen. With RT-PCR assay as the reference standard, this assay detected SARS-CoV-2 infection in 10 minutes, with the sensitivity, specificity, and percentage agreement of 75.6%, 100%, and 80.5%, respectively. A few limitations of the test were as follows :
(i) Small sample size
(ii) Insufficient patient medical information
(iii) Only symptomatic individuals were included
33031947
(Clin Microbiol Infect)
PMID
33031947
Date of Publishing: 2020 Oct 5
Title Accuracy of a nucleocapsid protein antigen rapid test in the diagnosis of SARS-CoV-2 infection
Author(s) nameDiao B, Wen K et al.
Journal Clin Microbiol Infect
Impact factor
6.17
Citation count: 9


This study reports a low-cost, quantitative paper-based electrochemical sensor chip for the digital detection of SARS-CoV-2 within 5 minutes. The biosensor consists of the ssDNA-conjugated AuNPs (gold nanoparticles) that simultaneously target two separate regions of the same SARS-CoV-2 N-gene. The study showed a sensitivity of 231 (copies µL-1)-1 and a limit of detection of 6.9 copies/µL. The sensor chip had several advantages over previously reported tests, an improved limit of detection, no need for additional redox medium for electron exchange, faster response to achieve stable data, excellent shelf life, and its plausible economic production.
33079516
(ACS Nano)
PMID
33079516
Date of Publishing: 2020 Oct 20
Title Rapid, Ultrasensitive, and quantitative detection of SARS-CoV-2 Using Antisense Oligonucletides Directed Electrochemical Biosensor Chip
Author(s) nameAlafeef M, Dighe K et al.
Journal ACS Nano
Impact factor
13.7
Citation count: 4


In this study, a simple electrochemical sensor-based diagnostic method is developed to detect the intensity of ROS levels in the sputum samples of candidates for early-stage screening of COVID-19. The assay resulted in accuracy and sensitivity of both 97% within 30 sec. The method provides an alternative to clinical judgment (HR-CT, ESR, CRP, CBC, Lymphopenia, and observational symptoms) and RT-PCR assays for fast screening of the patients who need a further medical examination.
32729548
(Biosens Bioelectron)
PMID
32729548
Date of Publishing: 2020 Oct 1
Title Real-time diagnosis of reactive oxygen species (ROS) in fresh sputum by electrochemical tracing; correlation between COVID-19 and viral-induced ROS in lung/respiratory epithelium during this pandemic.
Author(s) nameMiripour ZS, Sarrami-Forooshani R et al.
Journal Biosens Bioelectron
Impact factor
9.52
Citation count: 4


Reporting a real-time reverse-transcription recombinase-aided amplification assay (RT-RAA) specific for the N gene of SARS-CoV-2. The analytical specificity of the real-time RT-RAA assay was determined by the samples positive for other pathogens ( influenza A, influenza B, HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU1, respiratory syncytial virus, parainfluenza virus, rhinovirus, and human metapneumovirus), and the results were negative for all the samples. The kappa value of this assay was 0.959, representing no significant difference between both the assays.
32758712
(Virology)
PMID
32758712
Date of Publishing: 2020 Oct
Title A reverse-transcription recombinase-aided amplification assay for the rapid detection of N gene of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Author(s) nameWu T, Ge Y et al.
Journal Virology
Impact factor
2.819
Citation count: 4


Reporting a conventional two-step RT-PCR assay, specific for the E gene of SARS-CoV-2, which can be used as a lower-cost alternative method for the detection of SARS-CoV-2 in remote places. The conventional PCR detected until 1e-7 dilutions of the isolates. The highest Ct detected by conventional PCR was 35.98
32767275
(Braz J Microbiol)
PMID
32767275
Date of Publishing: 2020 Sep
Title Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR
Author(s) nameDorlass EG, Monteiro CO et al.
Journal Braz J Microbiol
Impact factor
1.67
Citation count: 2


Reporting a conventional one-step RT-PCR assay, specific for the E gene of SARS-CoV-2, which can be used as a lower-cost alternative method for the detection of SARS-CoV-2 in remote places. The conventional PCR detected until 1e-7 dilutions of the isolates. The highest Ct detected by conventional PCR was 35.98
32767275
(Braz J Microbiol)
PMID
32767275
Date of Publishing: 2020 Sep
Title Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR
Author(s) nameDorlass EG, Monteiro CO et al.
Journal Braz J Microbiol
Impact factor
1.67
Citation count: 2


Reporting a new single-tube nested real-time RT-PCR (STN RT-PCR) assay for the diagnosis of COVID-19 based on SARS-CoV-2 RdRp/Hel and N genes. This method was found highly sensitive and specific for SARS-CoV-2 detection. STN COVID-19-N assays is more sensitive than the non-nestedNassay.
32784770
(Int J Mol Sci)
PMID
32784770
Date of Publishing: 2020 Aug 7
Title Development and Evaluation of Novel and Highly Sensitive Single-Tube Nested Real-Time RT-PCR Assays for SARS-CoV-2 Detection
Author(s) nameYip CC, Sridhar S et al.
Journal Int J Mol Sci
Impact factor
4.21
Citation count: 2


Reporting DIOS RT-qPCR assay, which targets two regions of nucleocapsid genes (N1, N2) to detect SARS-CoV-2. Ct value of <40, was indicative of a SARS-CoV-2-positive sample. The pre-treatment of the swab sample by heat inactivation (75 degree Celsius for 10 min) was also tested. No significant changes in heat inactivation were observed. Comparative analysis of swab samples by DIOS-RT-qPCR (directly from a swab) and an IVD CE-certified RT-qPCR kit (using extracted RNA) was performed, 98% concordance results were observed.
32824767
(Diagnostics (Basel))
PMID
32824767
Date of Publishing: 2020 Aug 18
Title Direct-RT-qPCR detection of SARS-CoV-2 without RNA extraction as part of a Covid-19 testing strategy: From sample to result in one hour
Author(s) name Kriegova E, Fillerova R, Kvapil P.
Journal Diagnostics (Basel)
Impact factor
2.36
Citation count: 3


Reporting a low volume RT-RPA assay (real-time reverse transcription recombinase polymerase amplification), specific for the detection of N gene of SARS-CoV-2 by using an internally quenched exonuclease probe (exo-IQ). RT-RPA Assay is a novel candidate for use in microfluidic devices.
32384153
(Clin Chem)
PMID
32384153
Date of Publishing: 2020 Aug 1
Title Rapid Detection of SARS-CoV-2 by Low Volume Real-Time Single Tube Reverse Transcription Recombinase Polymerase Amplification Using an Exo Probe with an Internally Linked Quencher (Exo-IQ).
Author(s) nameBehrmann O, Bachmann I et al.
Journal Clin Chem
Impact factor
4.75
Citation count: 12


In this study, the Rapid Antigen detection kit, the BIOCREDIT COVID-19 Ag, was evaluated for diagnosing the SARS-CoV-2 virus in respiratory samples and compared with RT-PCR assay. This method was found less sensitive than RT-PCR. The kit produced false-negative results, and hence this test cannot individually diagnose a COVID-19 positive patient. Positive results are shown by appearance of black line in the result window of the kit.
Two major limitations of the test were the viral load variability prior to the test and the refrigeration of the samples for a week before testing.
32585619
(J Clin Virol)
PMID
32585619
Date of Publishing: 2020 Aug
Title Evaluation of rapid antigen test for detection of SARS-CoV-2 virus
Author(s) nameMak GC, Cheng PK et al.
Journal J Clin Virol
Impact factor
2.95
Citation count: 29


Reporting a new Hologic Panther APTIMA method based on transcription-mediated amplification assay, specifically designed to detect N1 and N2 genes of SARS-CoV-2. Higher analytical sensitivity of TMA compared to that of RT-PCR resulted in the determination of inconclusive result to a conclusive one.
32619959
(J Clin Virol)
PMID
32619959
Date of Publishing: 2020 Aug
Title High-Throughput Transcription-mediated amplification on the Hologic Panther is a highly sensitive method of detection for SARS-CoV-2
Author(s) nameGorzalski AJ, Tian H et al.
Journal J Clin Virol
Impact factor
2.95
Citation count: 5


Reporting one step nested RT-PCR, specific for ORF1ab gene, which performs two successive rounds of amplification at low cost with greater flexibility. Assay efficiency was found to be 99.66%. 183 bp sequence of ORF1ab gene (region 3801 to 3983 bp) of the reference genome (accession number: MN908947.3) has 100% similarity to all SARS-CoV-2 sequences reported in the GenBank, and 86.96% similarity with the bat SARS-like coronavirus isolate sequence. The 1COFw and 1CORw primers can be used separately for COVID-19 diagnosis from clinical samples.
32794453
(J Infect Dev Ctries)
PMID
32794453
Date of Publishing: 2020 Jul 31
Title One-step nested RT-PCR for COVID-19 detection: A flexible, locally developed test for SARS-CoV2 nucleic acid detection
Author(s) nameMeza-Robles C, Barajas-Saucedo CE et al.
Journal J Infect Dev Ctries
Impact factor
1.26
Citation count: 4