HC-FIA Assay


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Original Article
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Reporting an amplification-free, fluorescence-based diagnostic method, namely Hybrid Capture Fluorescence Immunoassay (HC-FIA), for diagnosing SARS-CoV-2. It is carried on a lateral flow strip and targets ORF1ab, E, and the N regions of the genome to detect SARS-CoV-2. The assay resulted in 100% and 99% sensitivities and specificities, respectively. This assay is based on the capture of RNA-DNA hybrids and on immunofluorescence analysis. The SARS-CoV-2 HC-FIA detection system Fig.1 (https://www.nature.com/articles/s41551-020-00655-z#Fig1)
33273714
(Nat Biomed Eng)
PMID
33273714
Date of Publishing: 2020 Dec
Title Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA
Author(s) nameWang D, He S et al.
Journal Nat Biomed Eng
Citation count 3