Droplet digital PCR assay(ddPCR)


Last updated: 2021 Jun 14
Total hit(s): 3
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Original Article
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In this research study, N- and E- gene multiplex (ddCoV_N and ddCoV_E) RT-ddPCR method is redesigned and optimized to detect SARS-CoV-2 from wastewater samples and compared with the standard nCoV_N2 and E_Sarbeco RT-qPCR assays. SARS-CoV-2 wastewater surveillance gives the community-level infection trends and screens infected individuals at the building level.
Differences within gene assays (ddCoV_N, ddCoV_E, and nCoV_N2, E_Sarbeco) were small because the primer and probe sequences were designed around the same genomic locations.
Pre-print (medRXiv)
Date of Publishing -
Title Redesigning SARS-CoV-2 clinical RT-qPCR assays for wastewater RT-ddPCR
Author(s) name -
Date of Entry 2021 Jun 14


Reporting one-step RT-dPCR (reverse transcription digital PCR ) method based on SARS-CoV-2 ORF1ab, N, and E gene for detecting SARS-CoV-2 from pharyngeal swab samples. RT dPCR uses droplets of samples with as low as 2 gene copies to diagnose COVID-19 positive. The Specificity of the assays were determined by testing Influenza virus and other human coronavirus and tests gave negative results.
33379001
(Talanta)
PMID
33379001
Date of Publishing: 2021 Mar 1
Title Highly accurate and sensitive diagnostic detection of SARS-CoV-2 by digital PCR
Author(s) nameDong L, Zhou J et al.
Journal Talanta
Citation count 2


Reporting a newly designed droplet digital PCR (ddPCR) assay, based on RNA-dependent RNA polymerase gene (RdRp) of SARS-CoV-2. This assay was extremely sensitive at detecting negative rtPCR samples (55 suspected SARS-CoV-2 rtPCR negative) as Positive. All positive detected clinical samples were characterized by low viral loads.
The positive SARS-CoV-2 samples detected by ddPCR were also confirmed by the Serological assay (presence of SARS-CoV-2 IgG) at the later stage of illness, results showed the presence of SARS-CoV-2 antibodies in all patients tested positive for SARS-CoV-2 in ddPCR (100%)
32898153
(PLoS One)
PMID
32898153
Date of Publishing: 2020
Title Detection and quantification of SARS-CoV-2 by droplet digital PCR in real-time PCR negative nasopharyngeal swabs from suspected COVID-19 patients
Author(s) nameAlteri C, Cento V et al.
Journal PLoS One
Impact factor
2.87
Citation count: 4