DIOS-RT-qPCR assay

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Reporting DIOS RT-qPCR assay, which targets two regions of nucleocapsid genes (N1, N2) to detect SARS-CoV-2. Ct value of <40, was indicative of a SARS-CoV-2-positive sample. The pre-treatment of the swab sample by heat inactivation (75 degree Celsius for 10 min) was also tested. No significant changes in heat inactivation were observed. Comparative analysis of swab samples by DIOS-RT-qPCR (directly from a swab) and an IVD CE-certified RT-qPCR kit (using extracted RNA) was performed, 98% concordance results were observed.
(Diagnostics (Basel))
Date of Publishing: 2020 Aug 18
Title Direct-RT-qPCR detection of SARS-CoV-2 without RNA extraction as part of a Covid-19 testing strategy: From sample to result in one hour
Author(s) name Kriegova E, Fillerova R, Kvapil P.
Journal Diagnostics (Basel)
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Citation count: 3