Sequence : Nsp1


Total row(s): 14
Select item(s)
Key Findings
Phylogenetic analysis of Israeli and Non-Israeli SARS-CoV-2 sequences revealed that the Israeli SARS-CoV-2 Strain containing P681H mutation originated from the B.1.1.50 Pangolin lineage.
PMID
Title A unique SARS-CoV-2 spike protein P681H strain detected in Israel
Author(s) nameNeta S. Zuckerman, Shay Fleishon, Efrat Bucris, Dana Bar-Ilan, Michal Linial, Itay Bar-Or, Victoria Indenbaum, Merav Weil,Ella Mendelson, Michal Mandelboim, Orna Mor
Journal
Impact factor
Citation count
Date of publishing2021 Mar 25
Metadata was analyzed to associate the frequent mutations and co-mutation patterns with COVID-19 patient status (deceased, symptomatic, mild, and asymptomatic groups).
PMID
Title Genomic surveillance and phylodynamic analyses reveal emergence of novel mutation and co-mutation patterns within SARS-CoV2 variants prevalent in India
Author(s) nameNupur Biswas, Priyanka Mallick, Sujay Krishna Maity, Debaleena Bhowmik, Arpita Ghosh Mitra, Soumen Saha, Aviral Roy, Partha Chakrabarti, Sandip Paul, Saikat Chakrabarti
Journal
Impact factor
Citation count
Date of publishing2021 Mar 25
The study reports a newly identified SARS-CoV-2 Strain from Israel. The strain included a non-synonymous mutation in the S protein: P681H (C23604A) and additional four synonymous mutations, Nsp3:C7765T, Nsp12b: C13821T, Nsp16:T21111C, and C29545A.
PMID
Title A unique SARS-CoV-2 spike protein P681H strain detected in Israel
Author(s) name-
Journal
Impact factor
Citation count
Date of publishing-
Mutation analysis of 2213 complete genomes from six geographical regions worldwide revealed 3178 polymorphic sites. Of these polymorphic sites, 58.5% (1861 sites) were non-synonymous, compared with the reference genome, Wuhan-Hu-1. Seven frequent non-synonymous mutations were observed in the global population of SARS-CoV-2.
PMID 33572190
Title Molecular Epidemiology Surveillance of SARS-CoV-2: Mutations and Genetic Diversity One Year after Emerging
Author(s) nameFlores-Alanis A, Cruz-Rangel A, Rodríguez-Gómez F, González J, Torres-Guerrero CA, Delgado G, Cravioto A, Morales-Espinosa R.
Journal Pathogens
Impact factor3.31
Citation count1
Date of publishing2021 Feb 9
62 mutations identified, including 30 mis-sense mutations, in 22 Moroccan patient isolates showed that Spike_D614G and NSP12_P323L mutations were present in all the analyzed sequences, whereas N_G204R and N_R203K were present in 9 sequences.
PMID 33558859
Title Genetic diversity and genomic epidemiology of SARS-CoV-2 in Morocco
Author(s) nameBadaoui B, Sadki K, Talbi C, Salah D, Tazi L.
Journal Biosaf Health
Impact factorCant find
Citation count1
Date of publishing2021 Feb 3
Most frequent and prevalent mutation reported, with reference to Wuhan sequence (hCoV-19/Wuhan/WIV04/2019), was P323L in the non-structural protein 12 (94.7%) whereas the second frequent mutation was D614G in the Spike glycoprotein region (92.6%), followed by G71S in the non-structural protein 5 (70%).
PMID 33359061
Title Molecular epidemiology of COVID-19 in Oman: A molecular andsurveillance study for the early transmission of COVID-19 in thecountry
Author(s) nameAl-Mahruqi S, Al-Wahaibi A, Khan AL, Al-Jardani A, Asaf S, Alkindi H, Al-Kharusi S, Al-Rawahi AN, Al-Rawahi A, Al-Salmani M, Al-Shukri I, Al-Busaidi A, Al-Abri SS, Al-Harrasi A.
Journal Int J Infect Dis
Impact factor3.42
Citation count1
Date of publishing2020 Dec 21
Primer pairs for a real-time RT-PCR test specific for the nsp1 gene of SARS-CoV-2.
PMID 32501535
Title Identification of nsp1 gene as the target of SARS-CoV-2 real time RT-PCR using nanopore whole genome sequencing
Author(s) nameChan WM, Ip JD, Chu AW, Yip CC, Lo LS, Chan KH, Ng AC, Poon RW, To WK, Tsang OT, Leung WS, Kwan MY, Chua GT, Chung TW, Hung IF, Kok KH, Cheng VC, Chan JF, Yuen KY, To KK.
Journal J Med Virol
Impact factor2.07
Citation count5
Date of publishing2020 Nov
Probe for a real-time RT-PCR test specific for the nsp1 gene of SARS-CoV-2.
PMID 32501535
Title Identification of nsp1 gene as the target of SARS-CoV-2 real time RT-PCR using nanopore whole genome sequencing
Author(s) nameChan WM, Ip JD, Chu AW, Yip CC, Lo LS, Chan KH, Ng AC, Poon RW, To WK, Tsang OT, Leung WS, Kwan MY, Chua GT, Chung TW, Hung IF, Kok KH, Cheng VC, Chan JF, Yuen KY, To KK.
Journal J Med Virol
Impact factor2.07
Citation count5
Date of publishing2020 Nov
Compared to the SARS-CoV-2 reference genome (GenBank accession number: MN996528.1), the SARS-CoV-2 genome of Bangladesh, BCSIR-NILMRC-006, (accession number: MT539159) had eight mutations. A unique mutation was observed in the non-structural protein NSP2 of the ORF1ab gene.
PMID 32972934
Title Coding-Complete Genome Sequences of Three SARS-CoV-2 Strains from Bangladesh
Author(s) nameAkter S, Banu TA, Goswami B, Osman E, Uzzaman MS, Habib MA, Jahan I, Mahmud ASM, Sarker MMH, Hossain MS, Shamsuzzaman AKM, Nafisa T, Molla MMA, Yeasmin M, Ghosh AK, Al Din SMS, Ray UC, Sajib SA, Hossain M, Khan MS.
Journal Microbiol Resour Announc
Impact factor0.88
Citation count1
Date of publishing2020 Sep 24
Compared to the SARS-CoV-2 reference genome (GenBank accession number: MN996528.1), the SARS-CoV-2 genome of Bangladesh, BCSIR-NILMRC-007, (accession number: MT539158) had six mutations.
PMID 32972934
Title Coding-Complete Genome Sequences of Three SARS-CoV-2 Strains from Bangladesh
Author(s) nameAkter S, Banu TA, Goswami B, Osman E, Uzzaman MS, Habib MA, Jahan I, Mahmud ASM, Sarker MMH, Hossain MS, Shamsuzzaman AKM, Nafisa T, Molla MMA, Yeasmin M, Ghosh AK, Al Din SMS, Ray UC, Sajib SA, Hossain M, Khan MS.
Journal Microbiol Resour Announc
Impact factor0.88
Citation count1
Date of publishing2020 Sep 24
Compared to the SARS-CoV-2 reference genome (GenBank accession number: MN996528.1), the SARS-CoV-2 genome of Bangladesh, BCSIR-NILMRC-008, (accession number: MT539160) had six mutations.
PMID 32972934
Title Coding-Complete Genome Sequences of Three SARS-CoV-2 Strains from Bangladesh
Author(s) nameAkter S, Banu TA, Goswami B, Osman E, Uzzaman MS, Habib MA, Jahan I, Mahmud ASM, Sarker MMH, Hossain MS, Shamsuzzaman AKM, Nafisa T, Molla MMA, Yeasmin M, Ghosh AK, Al Din SMS, Ray UC, Sajib SA, Hossain M, Khan MS.
Journal Microbiol Resour Announc
Impact factor0.88
Citation count1
Date of publishing2020 Sep 24
Mutation analysis of 2492 SARS-CoV-2 genomes (belonging to 58 different countries representing six continents and five different climatic zones) revealed 1516 nucleotide variations, 744 amino acid substitutions, and 12 deletion sites. The pattern of mutations was different among the six different continents (Europe, Asia, North America, South America, Africa, and Australia) and five different climatic zones (temperate, tropical, diverse, dry, and continental).
PMID 32814791
Title Genome-wide analysis of SARS-CoV-2 virus strains circulating worldwide implicates heterogeneity
Author(s) nameIslam MR, Hoque MN, Rahman MS, Alam ASMRU, Akther M, Puspo JA, Akter S, Sultana M, Crandall KA, Hossain MA.
Journal Sci Rep
Impact factor4.12
Citation count17
Date of publishing2020 Aug 19
Sequence alignment of SARSCoV2 Nsp15 coronaviral homologs with SARSCoV2, SARSCoV, MERSCoV, HCoV229E, MHV
PMID 32304108
Title Crystal structure of Nsp15 endoribonuclease NendoU from SARSCoV2
Author(s) nameKim Y, Jedrzejczak R, Maltseva NI, Wilamowski M, Endres M, Godzik A, Michalska K, Joachimiak A.
Journal Protein Sci
Impact factor2.4
Citation count58
Date of publishing2020 Jul
Compared to the SARS-CoV-2 reference genome (MN908947.3), the SARS-CoV-2 isolate, CHRF_nCoV19_0001/Bangladesh (accession number: MT476385), was observed to have nine mutations.
PMID 32527780
Title Complete Genome Sequence of a Novel Coronavirus (SARS-CoV-2) Isolate from Bangladesh
Author(s) nameSaha S, Malaker R, Sajib MSI, Hasanuzzaman M, Rahman H, Ahmed ZB, Islam MS, Islam M, Hooda Y, Ahyong V, Vanaerschot M, Batson J, Hao S, Kamm J, Kistler A, Tato CM, DeRisi JL, Saha SK.
Journal Microbiol Resour Announc
Impact factor0.88
Citation count4
Date of publishing2020 Jun 11

Structure : Nsp1


Total row(s): 8
Select item(s)
Key Findings
Structural characterisation of SARS-CoV-2 mini replication and transcription complex
PMID 33208736
Title Architecture of a SARS-CoV-2 mini replication and transcription complex
Author(s) nameYan L, Zhang Y, Ge J, Zheng L, Gao Y, Wang T, Jia Z, Wang H, Huang Y, Li M, Wang Q, Rao Z, Lou Z.
Journal Nat Commun
Impact factor11.8
Citation count4
Date of publishing2020 Nov 18
Structural characterisation of SARS-CoV-2 mini replication and transcription complex
PMID 33208736
Title Architecture of a SARS-CoV-2 mini replication and transcription complex
Author(s) nameYan L, Zhang Y, Ge J, Zheng L, Gao Y, Wang T, Jia Z, Wang H, Huang Y, Li M, Wang Q, Rao Z, Lou Z.
Journal Nat Commun
Impact factor11.8
Citation count4
Date of publishing2020 Nov 18
Structural characterization of SARS-CoV-2 nsp10 in its unbound form and its behavior in a liquid.
PMID 33036230
Title Crystal Structure of Non-Structural Protein 10 from Severe Acute Respiratory Syndrome Coronavirus-2
Author(s) nameRogstam A, Nyblom M, Christensen S, Sele C, Talibov VO, Lindvall T, Rasmussen AA, André I, Fisher Z, Knecht W, Kozielski F.
Journal Int J Mol Sci
Impact factor4.21
Citation count1
Date of publishing2020 Oct 6
Structure of SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) complex in its active form consisting of nsp12, nsp8, nsp7, and duplex RNA.
PMID 32438371
Title Structure of replicating SARS-CoV-2 polymerase
Author(s) nameHillen HS, Kokic G, Farnung L, Dienemann C, Tegunov D, Cramer P.
Journal Nature
Impact factor24.36
Citation count74
Date of publishing2020 Aug
Structural characterization of NSP15 Endoribonuclease from SARS CoV-2
PMID 32304108
Title Crystal structure of Nsp15 endoribonuclease NendoU from SARSCoV2
Author(s) nameKim Y, Jedrzejczak R, Maltseva NI, Wilamowski M, Endres M, Godzik A, Michalska K, Joachimiak A.
Journal Protein Sci
Impact factor2.4
Citation count58
Date of publishing2020 Jul
Structural characterization of NSP15 Endoribonuclease from SARS CoV-2 in the complex with a citrate
PMID 32304108
Title Crystal structure of Nsp15 endoribonuclease NendoU from SARSCoV2
Author(s) nameKim Y, Jedrzejczak R, Maltseva NI, Wilamowski M, Endres M, Godzik A, Michalska K, Joachimiak A.
Journal Protein Sci
Impact factor2.4
Citation count58
Date of publishing2020 Jul
Structural characterization of cryo-EM structure of the apo nsp12-nsp7-nsp8 complex
PMID 32358203
Title Structural basis for inhibition of the RNA-dependent RNA polymerase from SARS-CoV-2 by remdesivir
Author(s) nameYin W, Mao C, Luan X, Shen DD, Shen Q, Su H, Wang X, Zhou F, Zhao W, Gao M, Chang S, Xie YC, Tian G, Jiang HW, Tao SC, Shen J, Jiang Y, Jiang H, Xu Y, Zhang S, Zhang Y, Xu HE.
Journal Science
Impact factor20.57
Citation count156
Date of publishing2020 Jun 26
Structural characteirzation of the nsp12-nsp7-nsp8 complex bound to the template-primer RNA and triphosphate form of Remdesivir(RTP)
PMID 32358203
Title Structural basis for inhibition of the RNA-dependent RNA polymerase from SARS-CoV-2 by remdesivir
Author(s) nameYin W, Mao C, Luan X, Shen DD, Shen Q, Su H, Wang X, Zhou F, Zhao W, Gao M, Chang S, Xie YC, Tian G, Jiang HW, Tao SC, Shen J, Jiang Y, Jiang H, Xu Y, Zhang S, Zhang Y, Xu HE.
Journal Science
Impact factor20.57
Citation count156
Date of publishing2020 Jun 26

Drugs : Nsp1


Total row(s): 4
Select item(s)
Key Findings
Virulence protein factor Nsp1 was targeted by in silico virtual screening of ligand libraries. Molecular docking simulations of the top6 screened ligands with Nsp1 were used and the ligand-Nsp1 complexes were subjected to molecular dynamics simulations to analyze the behaviours of the ligands in a virtual cell
PMID 33612076
Title Identification of the binding interactions of some novel antiviral compounds against Nsp1 protein from SARS-CoV-2 (COVID-19) through high throughput screening.
Author(s) nameChowdhury N, Bagchi A.
Journal J Biomol Struct Dyn
Impact factor3.22
Citation count1
Date of publishing2021 Feb 22
In-silico models of Nsp13 helicase and nsp14 were generated using comparative homology modelling. The structures were validated and then used for virtual screening of pre-existing, FDA approved antiviral drugs.This was done to verify that these drugs could be repurposed to be used against SARS-CoV-2 infection.
PMID 32875166
Title In silico structure modelling of SARS-CoV-2 Nsp13 helicase and Nsp14 and repurposing of FDA approved antiviral drugs as dual inhibitors
Author(s) nameGurung AB.
Journal Gene Rep
Impact factor0.61
Citation count5
Date of publishing2020 Dec
Virtual screening of 970 000 chemical compounds against the ATP-binding site to identify potential inhibitors indicates two of the top drug hits (Cepharanthine,Lumacaftor) have significant activity in inhibiting purified recombinant SARS-CoV-2 helicase.
PMID 33052685
Title Discovery of COVID-19 Inhibitors Targeting the SARS-CoV-2 Nsp13 Helicase
Author(s) nameWhite MA, Lin W, Cheng X.
Journal J Phys Chem Lett
Impact factor7.33
Citation count2
Date of publishing2020 Nov 5
The study showed that SARS-CoV-2 NSP14-a bifunctional enzyme ,could be a potential drug target for intervention and four drugs-Saquinavir, Hypericin, Baicalein and Bromocriptine were seen to be able to bind to the N-terminal and C-terminal domains of SARS-CoV-2 NSP 14 were seen as potential drug targets for intervention.
PMID 32923004
Title Potential Treatment of Chinese and Western Medicine Targeting Nsp14 of SARS-CoV-2
Author(s) nameLiu C, Zhu X, Lu Y, Zhang X, Jia X, Yang T.
Journal J Pharm Anal
Impact factor4.84
Citation count3
Date of publishing2020 Sep 7

Diagnostics : Nsp1


Total row(s): 1
Select item(s)
Key Findings
Reporting a novel nsp1 real-time RT-PCR assay for the detection of SARS-CoV-2, based on nanopore sequencing.
PMID 32501535
Title Identification of nsp1 gene as the target of SARS-CoV-2 real time RT-PCR using nanopore whole genome sequencing
Author(s) nameChan WM, Ip JD, Chu AW, Yip CC, Lo LS, Chan KH, Ng AC, Poon RW, To WK, Tsang OT, Leung WS, Kwan MY, Chua GT, Chung TW, Hung IF, Kok KH, Cheng VC, Chan JF, Yuen KY, To KK.
Journal J Med Virol
Impact factor2.07
Citation count5
Date of publishing2020 Nov

Molecular_interactions : Nsp1


Total row(s): 3
Select item(s)
Key Findings
Molecular interaction study of nsp13-2 and template RNA.
PMID 33208736
Title Architecture of a SARS-CoV-2 mini replication and transcription complex
Author(s) nameYan L, Zhang Y, Ge J, Zheng L, Gao Y, Wang T, Jia Z, Wang H, Huang Y, Li M, Wang Q, Rao Z, Lou Z.
Journal Nat Commun
Impact factor11.8
Citation count4
Date of publishing2020 Nov 18
Structural characterization of SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) complex in its active form consisting of nsp12, nsp8, nsp7 and duplex RNA.
PMID 32438371
Title Structure of replicating SARS-CoV-2 polymerase
Author(s) nameHillen HS, Kokic G, Farnung L, Dienemann C, Tegunov D, Cramer P.
Journal Nature
Impact factor24.36
Citation count74
Date of publishing2020 Aug
Molecular interactions of SARS-CoV-2 RdRp in complex with a 50-base template-primer RNA and remdesivir.
PMID 32358203
Title Structural basis for inhibition of the RNA-dependent RNA polymerase from SARS-CoV-2 by remdesivir
Author(s) nameYin W, Mao C, Luan X, Shen DD, Shen Q, Su H, Wang X, Zhou F, Zhao W, Gao M, Chang S, Xie YC, Tian G, Jiang HW, Tao SC, Shen J, Jiang Y, Jiang H, Xu Y, Zhang S, Zhang Y, Xu HE.
Journal Science
Impact factor20.57
Citation count156
Date of publishing2020 Jun 26