In vitro studies


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Key Findings
The in vitro mutation analysis showed that the D614G mutation changes the spike protein sorting, enhances the trafficking of spike protein to the lysosome.
PMID 33330866
Title The D614G Mutation Enhances the Lysosomal Trafficking of SARS-CoV-2 Spike
Author(s) nameGuo C, Tsai SJ, Ai Y, Li M, Pekosz A, Cox A, Atai N, Gould SJ.
Journal bioRxiv
Impact factorIts not a journal
Citation count1
Date of publishing2020 Dec 9
To understand the mechanism of lysosomal trafficking of the spike protein, the Htet1/SD614G cells were incubated with doxycycline.
PMID 33330866
Title The D614G Mutation Enhances the Lysosomal Trafficking of SARS-CoV-2 Spike
Author(s) nameGuo C, Tsai SJ, Ai Y, Li M, Pekosz A, Cox A, Atai N, Gould SJ.
Journal bioRxiv
Impact factorIts not a journal
Citation count1
Date of publishing2020 Dec 9
To understand the molecular mechanism of lysosomal trafficking of the spike protein, the Htet1/SD614G cells were incubated with doxycycline and dynasore.
PMID 33330866
Title The D614G Mutation Enhances the Lysosomal Trafficking of SARS-CoV-2 Spike
Author(s) nameGuo C, Tsai SJ, Ai Y, Li M, Pekosz A, Cox A, Atai N, Gould SJ.
Journal bioRxiv
Impact factorIts not a journal
Citation count1
Date of publishing2020 Dec 9
To understand the molecular mechanism of lysosomal trafficking of the spike protein, the Htet1/SD614G cells were incubated with doxycycline and pitstop2.
PMID 33330866
Title The D614G Mutation Enhances the Lysosomal Trafficking of SARS-CoV-2 Spike
Author(s) nameGuo C, Tsai SJ, Ai Y, Li M, Pekosz A, Cox A, Atai N, Gould SJ.
Journal bioRxiv
Impact factorIts not a journal
Citation count1
Date of publishing2020 Dec 9
To understand the molecular mechanism of lysosomal trafficking of the spike protein, the Htet1/SD614G cells were incubated with nocodazole.
PMID 33330866
Title The D614G Mutation Enhances the Lysosomal Trafficking of SARS-CoV-2 Spike
Author(s) nameGuo C, Tsai SJ, Ai Y, Li M, Pekosz A, Cox A, Atai N, Gould SJ.
Journal bioRxiv
Impact factorIts not a journal
Citation count1
Date of publishing2020 Dec 9
To understand the molecular mechanism of lysosomal trafficking of the spike protein, the Htet1/SD614G cells were incubated with bafilomycin A1.
PMID 33330866
Title The D614G Mutation Enhances the Lysosomal Trafficking of SARS-CoV-2 Spike
Author(s) nameGuo C, Tsai SJ, Ai Y, Li M, Pekosz A, Cox A, Atai N, Gould SJ.
Journal bioRxiv
Impact factorIts not a journal
Citation count1
Date of publishing2020 Dec 9
The Growth properties of Del-mut-1 variant and WT viruses in Vero-E6, A549, Caco-2, and Calu-3 cells showed stability and increased population along the passages only in the Vero-E6 cells
PMID 32301390
Title Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction
Author(s) nameLau SY, Wang P, Mok BW, Zhang AJ, Chu H, Lee AC, Deng S, Chen P, Chan KH, Song W, Chen Z, To KK, Chan JF, Yuen KY, Chen H.
Journal Emerg Microbes Infect
Impact factor5.84
Citation count36
Date of publishing2020 Dec
The in vitro mutation analysis of the D614G mutation showed no effect on viral replication and viral infectivity in Vero E6 cells.
PMID 33106671
Title Spike mutation D614G alters SARS-CoV-2 fitness
Author(s) namePlante JA, Liu Y, Liu J, Xia H, Johnson BA, Lokugamage KG, Zhang X, Muruato AE, Zou J, Fontes-Garfias CR, Mirchandani D, Scharton D, Bilello JP, Ku Z, An Z, Kalveram B, Freiberg AN, Menachery VD, Xie X, Plante KS, Weaver SC, Shi PY.
Journal Nature
Impact factor24.36
Citation count38
Date of publishing2020 Oct 26
Compared with the wild D614 virus, the mutant G614 virus showed increased viral replication and viral infectivity in the cell line Calu-3.
PMID 33106671
Title Spike mutation D614G alters SARS-CoV-2 fitness
Author(s) namePlante JA, Liu Y, Liu J, Xia H, Johnson BA, Lokugamage KG, Zhang X, Muruato AE, Zou J, Fontes-Garfias CR, Mirchandani D, Scharton D, Bilello JP, Ku Z, An Z, Kalveram B, Freiberg AN, Menachery VD, Xie X, Plante KS, Weaver SC, Shi PY.
Journal Nature
Impact factor24.36
Citation count38
Date of publishing2020 Oct 26
Compared with the wild D614 virus, the mutant G614 virus showed increased viral replication and viral infectivity in the primary human airway tissue culture model.
PMID 33106671
Title Spike mutation D614G alters SARS-CoV-2 fitness
Author(s) namePlante JA, Liu Y, Liu J, Xia H, Johnson BA, Lokugamage KG, Zhang X, Muruato AE, Zou J, Fontes-Garfias CR, Mirchandani D, Scharton D, Bilello JP, Ku Z, An Z, Kalveram B, Freiberg AN, Menachery VD, Xie X, Plante KS, Weaver SC, Shi PY.
Journal Nature
Impact factor24.36
Citation count38
Date of publishing2020 Oct 26
The SARS-CoV-2 GZ69 isolate from Brescia (Italy), showed no cytopathic effect on Vero E6 cells despite having a high viral load in the cell culture. The SARS-CoV-2 AP66 isolate induced the cytopathic effect.
PMID 32967693
Title A persistently replicating SARS-CoV-2 variant derived from an asymptomatic individual
Author(s) nameCaccuri F, Zani A, Messali S, Giovanetti M, Bugatti A, Campisi G, Filippini F, Scaltriti E, Ciccozzi M, Fiorentini S, Caruso A.
Journal J Transl Med
Impact factor4.2
Citation count4
Date of publishing2020 Sep 23
The SARS-CoV-2 isolates showed cytopathic effects on Vero E6 at 72 h post-inoculation and reached 90% of the cells at 5th days post-infection (dpi).
PMID 33015402
Title Characterization of local SARS-CoV-2 isolates and pathogenicity in IFNAR-/-mice
Author(s) nameHanifehnezhad A, Kehribar EŞ, Öztop S, Sheraz A, Kasırga S, Ergünay K, Önder S, Yılmaz E, Engin D, Oğuzoğlu TÇ, Şeker UÖŞ, Yılmaz E, Özkul A.
Journal Heliyon
Impact factor1.65
Citation count3
Date of publishing2020 Sep
The in vitro analysis of the deletion variants revealed that Var1 (deletion of QTQTN in spike protein) reduced the viral replication during late-phase, and Var2 (deletion of NSPRRAR in spike protein) didnt affect viral replication in Vero and Vero-E6 cells.
PMID 32571797
Title Identification of Common Deletions in the Spike Protein of Severe Acute Respiratory Syndrome Coronavirus 2
Author(s) nameLiu Z, Zheng H, Lin H, Li M, Yuan R, Peng J, Xiong Q, Sun J, Li B, Wu J, Yi L, Peng X, Zhang H, Zhang W, Hulswit RJG, Loman N, Rambaut A, Ke C, Bowden TA, Pybus OG, Lu J.
Journal J Virol
Impact factor4.16
Citation count12
Date of publishing2020 Aug 17
The SARS-CoV-2 isolates showed Cytopathogenic in Vero E6 and Huh7 cells at three days post-infection.
PMID 32015507
Title A pneumonia outbreak associated with a new coronavirus of probable bat origin
Author(s) nameZhou P, Yang XL, Wang XG, Hu B, Zhang L, Zhang W, Si HR, Zhu Y, Li B, Huang CL, Chen HD, Chen J, Luo Y, Guo H, Jiang RD, Liu MQ, Chen Y, Shen XR, Wang X, Zheng XS, Zhao K, Chen QJ, Deng F, Liu LL, Yan B, Zhan FX, Wang YY, Xiao GF, Shi ZL.
Journal Nature
Impact factor24.36
Citation count3984
Date of publishing2020 Mar