Single-tube nested (STN) RT-qPCR

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Key Findings
Reporting a new single-tube nested real-time RT-PCR (STN RT-PCR) assay for the diagnosis of COVID-19 based on SARS-CoV-2 RdRp/Hel and N genes. This method was found highly sensitive and specific for SARS-CoV-2 detection.
PMID 32784770
Title Development and Evaluation of Novel and Highly Sensitive Single-Tube Nested Real-Time RT-PCR Assays for SARS-CoV-2 Detection
Author(s) nameYip CC, Sridhar S, Leung KH, Ng AC, Chan KH, Chan JF, Tsang OT, Hung IF, Cheng VC, Yuen KY, To KK.
Journal Int J Mol Sci
Impact factor4.21
Citation count2
Date of publishing2020 Aug 7