RT-qPCR assay


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Reporting a novel nsp1 real-time RT-PCR assay for the detection of SARS-CoV-2, based on nanopore sequencing.
PMID 32501535
Title Identification of nsp1 gene as the target of SARS-CoV-2 real time RT-PCR using nanopore whole genome sequencing
Author(s) nameChan WM, Ip JD, Chu AW, Yip CC, Lo LS, Chan KH, Ng AC, Poon RW, To WK, Tsang OT, Leung WS, Kwan MY, Chua GT, Chung TW, Hung IF, Kok KH, Cheng VC, Chan JF, Yuen KY, To KK.
Journal J Med Virol
Impact factor2.07
Citation count5
Date of publishing2020 Nov
In this study, the RT-PCR assays were conducted using three protocols, CDC, NIID, and YCH assay for the diagnosis of SARS-CoV-2. The YCH assay targets 2 sites of the N gene (YCH-N1, and YCH-N2) and utilizes double-quencher probes. The double quencher probe reduced the background signal and detected SARS-CoV-2 in the low viral load clinical specimens.
PMID 32650037
Title Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay
Author(s) nameHirotsu Y, Mochizuki H, Omata M.
Journal J Virol Methods
Impact factor1.76
Citation count9
Date of publishing2020 Oct
In this study, the RT-PCR assays were conducted using three protocols, CDC, NIID, and YCH assay for the diagnosis of SARS-CoV-2. The NIID assay (Japan) targets 2 sites of the N gene (NIID-N1, NIID-N2) and utilizes single-quencher probes. The positive detection rate of N2 was higher than that of the N1 site.
PMID 32650037
Title Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay
Author(s) nameHirotsu Y, Mochizuki H, Omata M.
Journal J Virol Methods
Impact factor1.76
Citation count9
Date of publishing2020 Oct
In this study, the RT-PCR assays were conducted using three protocols, CDC, NIID, and YCH assay for the diagnosis of SARS-CoV-2. The CDC (USA) protocol targets 3 sites of the N gene (CDC-N1, CDC-N2, CDC-N3) and utilizes single-quencher probes. The CDC-N2 assay had a limit of detection of 10 copies of the DNA positive control.
PMID 32650037
Title Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay
Author(s) nameHirotsu Y, Mochizuki H, Omata M.
Journal J Virol Methods
Impact factor1.76
Citation count9
Date of publishing2020 Oct
In this study, a real-time RT-PCR assay was assessed by a sample pooling strategy. The study revealed that pooling of up to 10 samples per pool increased test capacity, with 60% of resource-saving, and detected positive samples with sufficient diagnostic accuracy. Ct values were lower in retested positive individual samples compared with pools.
PMID 32869865
Title Evaluation of sample pooling for diagnosis of COVID- 19 by real-time PCR : A resource-saving combat stratergy
Author(s) nameGarg J, Singh V, Pandey P, Verma A, Sen M, Das A, Agarwal J.
Journal J Med Virol
Impact factor2.07
Citation count4
Date of publishing2020 Sep 1
Reporting a one-step SYBR Green-based RT-qPCR assay specific for the E gene of SARS-CoV-2, which can be used as a lower-cost alternative to TaqMan RT-qPCR assay.
PMID 32767275
Title Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR
Author(s) nameDorlass EG, Monteiro CO, Viana AO, Soares CP, Machado RRG, Thomazelli LM, Araujo DB, Leal FB, Candido ED, Telezynski BL, Valério CA, Chalup VN, Mello R, Almeida FJ, Aguiar AS, Barrientos ACM, Sucupira C, De Paulis M, Sáfadi MAP, Silva DGBP, Sodré JJM, Soledade MP, Matos SF, Ferreira SR, Pinez CMN, Buonafine CP, Pieroni LNF, Malta FM, Santana RAF, Souza EC, Fock RA, Pinho JRR, Ferreira LCS, Botosso VF, Durigon EL, Oliveira DBL.
Journal Braz J Microbiol
Impact factor1.67
Citation count2
Date of publishing2020 Sep
Reporting a two-step SYBR Green-based RT-qPCR assay specific for the E gene of SARS-CoV-2, which can be used as a lower-cost alternative to TaqMan RT-qPCR assay.
PMID 32767275
Title Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR
Author(s) nameDorlass EG, Monteiro CO, Viana AO, Soares CP, Machado RRG, Thomazelli LM, Araujo DB, Leal FB, Candido ED, Telezynski BL, Valério CA, Chalup VN, Mello R, Almeida FJ, Aguiar AS, Barrientos ACM, Sucupira C, De Paulis M, Sáfadi MAP, Silva DGBP, Sodré JJM, Soledade MP, Matos SF, Ferreira SR, Pinez CMN, Buonafine CP, Pieroni LNF, Malta FM, Santana RAF, Souza EC, Fock RA, Pinho JRR, Ferreira LCS, Botosso VF, Durigon EL, Oliveira DBL.
Journal Braz J Microbiol
Impact factor1.67
Citation count2
Date of publishing2020 Sep
Comparative study of diagnosis of COVID-19 by chest CT scans and RT-PCR assay showed that chest CT scans for the primary screening of COVID-19 showed a low positive predictive value than RT-PCR assay. For chest CT scans and RT-PCR, the Positive predictive value ranged from 1.5%-30.7% and 47.3%-96.4%, respectively.
PMID 32301646
Title Diagnostic Performance of CT and Reverse Transcriptase-Polymerase Chain Reaction for Coronavirus Disease 2019: A Meta-Analysis
Author(s) nameKim H, Hong H, Yoon SH.
Journal Radiology
Impact factor7.11
Citation count120
Date of publishing2020 Sep
Reporting a rapid, inexpensive method, Direct RT-qPCR to detect SARS-CoV-2 from all respiratory specimens by targeting the E gene. The assay had a detection rate of 95.8 % for Ct values <35.
PMID 32795959
Title Extraction-free SARS-CoV-2 detection by rapid RT-qPCR universal for all primary respiratory materials
Author(s) nameLübke N, Senff T, Scherger S, Hauka S, Andrée M, Adams O, Timm J, Walker A.
Journal J Clin Virol
Impact factor2.95
Citation count4
Date of publishing2020 Sep
Reporting DIOS RT-qPCR assay, which targets two regions of nucleocapsid genes (N1, N2) to detect SARS-CoV-2.
PMID 32824767
Title Direct-RT-qPCR detection of SARS-CoV-2 without RNA extraction as part of a Covid-19 testing strategy: From sample to result in one hour
Author(s) nameKriegova E, Fillerova R, Kvapil P.
Journal Diagnostics (Basel)
Impact factor2.36
Citation count3
Date of publishing2020 Aug 18
Reporting diagnostic panel developed by US CDC consists of 3 real-time reverse transcription PCR assays specific for detecting the nucleocapsid gene of SARS-COV-2.
PMID 32396505
Title US CDC Real-Time Reverse Transcription PCR Panel for detection of Severe Acute Respiratory Syndrome Coronavirus 2
Author(s) nameLu X, Wang L, Sakthivel SK, Whitaker B, Murray J, Kamili S, Lynch B, Malapati L, Burke SA, Harcourt J, Tamin A, Thornburg NJ, Villanueva JM, Lindstrom S.
Journal Emerg Infect Dis
Impact factor6.81
Citation count38
Date of publishing2020 Aug
The implementation of real-time RT-PCR for the detection of COVID-19 based on SARS-CoV-2 nucleocapsid gene set 1 and 2.
PMID 32074516
Title Development of genetic diagnostic methods for detection for Novel Coronavirus 2019 (nCoV-2019) in Japan
Author(s) nameShirato K, Nao N, Katano H, Takayama I, Saito S, Kato F, Katoh H, Sakata M, Nakatsu Y, Mori Y, Kageyama T, Matsuyama S, Takeda M.
Journal Jpn J Infect Dis
Impact factor1.11
Citation count72
Date of publishing2020 Jul 22
SARS-CoV-2 was detected, from feces specimens, by using the qRT-PCR method.
PMID 32159775
Title Detection of SARS-CoV-2 in different types of clinical specimens
Author(s) nameWang W, Xu Y, Gao R, Lu R, Han K, Wu G, Tan W.
Journal JAMA
Impact factor14.78
Citation count1133
Date of publishing2020 May 12
SARS-CoV-2 was detected, from urine specimens, by using the qRT-PCR method.
PMID 32159775
Title Detection of SARS-CoV-2 in different types of clinical specimens
Author(s) nameWang W, Xu Y, Gao R, Lu R, Han K, Wu G, Tan W.
Journal JAMA
Impact factor14.78
Citation count1133
Date of publishing2020 May 12
SARS-CoV-2 was detected, from blood specimens, by using the qRT-PCR method.
PMID 32159775
Title Detection of SARS-CoV-2 in different types of clinical specimens
Author(s) nameWang W, Xu Y, Gao R, Lu R, Han K, Wu G, Tan W.
Journal JAMA
Impact factor14.78
Citation count1133
Date of publishing2020 May 12
SARS-CoV-2 was detected, from pharyngeal swab specimens, by using the qRT-PCR method.
PMID 32159775
Title Detection of SARS-CoV-2 in different types of clinical specimens
Author(s) nameWang W, Xu Y, Gao R, Lu R, Han K, Wu G, Tan W.
Journal JAMA
Impact factor14.78
Citation count1133
Date of publishing2020 May 12
SARS-CoV-2 was detected, from nasal swab specimens, by using the qRT-PCR method.
PMID 32159775
Title Detection of SARS-CoV-2 in different types of clinical specimens
Author(s) nameWang W, Xu Y, Gao R, Lu R, Han K, Wu G, Tan W.
Journal JAMA
Impact factor14.78
Citation count1133
Date of publishing2020 May 12
SARS-CoV-2 was detected, from sputum samples, by using the qRT-PCR method.
PMID 32159775
Title Detection of SARS-CoV-2 in different types of clinical specimens
Author(s) nameWang W, Xu Y, Gao R, Lu R, Han K, Wu G, Tan W.
Journal JAMA
Impact factor14.78
Citation count1133
Date of publishing2020 May 12
SARS-CoV-2 was detected, from fibrobronchoscope brush biopsy specimens, by using the qRT-PCR method.
PMID 32159775
Title Detection of SARS-CoV-2 in different types of clinical specimens
Author(s) nameWang W, Xu Y, Gao R, Lu R, Han K, Wu G, Tan W.
Journal JAMA
Impact factor14.78
Citation count1133
Date of publishing2020 May 12
SARS-CoV-2 was detected, from Bronchoalveolar lavage fluid samples, by using the qRT-PCR method.
PMID 32159775
Title Detection of SARS-CoV-2 in different types of clinical specimens
Author(s) nameWang W, Xu Y, Gao R, Lu R, Han K, Wu G, Tan W.
Journal JAMA
Impact factor14.78
Citation count1133
Date of publishing2020 May 12
Reporting a RT-qPCR assay specific for ORF 1b and N gene of SARS-CoV-2 and closely related species.
PMID 32031583
Title Molecular Diagnosis of a Novel Coronavirus (2019-nCoV) Causing an Outbreak of Pneumonia
Author(s) nameChu DKW, Pan Y, Cheng SMS, Hui KPY, Krishnan P, Liu Y, Ng DYM, Wan CKC, Yang P, Wang Q, Peiris M, Poon LLM.
Journal Clin Chem
Impact factor4.75
Citation count259
Date of publishing2020 Apr 1
Reporting a qPCR-based detection method specific for the detection of the receptor-binding domain of the S gene of SARS-CoV-2.
PMID 32015507
Title A pneumonia outbreak associated with a new coronavirus of probable bat origin
Author(s) nameZhou P, Yang XL, Wang XG, Hu B, Zhang L, Zhang W, Si HR, Zhu Y, Li B, Huang CL, Chen HD, Chen J, Luo Y, Guo H, Jiang RD, Liu MQ, Chen Y, Shen XR, Wang X, Zheng XS, Zhao K, Chen QJ, Deng F, Liu LL, Yan B, Zhan FX, Wang YY, Xiao GF, Shi ZL.
Journal Nature
Impact factor24.36
Citation count3984
Date of publishing2020 Mar
Reporting RT-qPCR N gene assay which, can be used as the additional confirmatory assay for SARS-CoV-2 detection.
PMID 31992387
Title Detection of Covid 19 novel coronavirus (2019 -nCoV) by real-time RT-PCR
Author(s) nameCorman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DK, Bleicker T, Brünink S, Schneider J, Schmidt ML, Mulders DG, Haagmans BL, van der Veer B, van den Brink S, Wijsman L, Goderski G, Romette JL, Ellis J, Zambon M, Peiris M, Goossens H, Reusken C, Koopmans MP, Drosten C.
Journal Euro Surveill
Impact factor7.37
Citation count1206
Date of publishing2020 Jan
Reporting RT-qPCR RdRP gene assay which, can be used as the confirmatory test for SARS-CoV-2 detection.
PMID 31992387
Title Detection of Covid 19 novel coronavirus (2019 -nCoV) by real-time RT-PCR
Author(s) nameCorman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DK, Bleicker T, Brünink S, Schneider J, Schmidt ML, Mulders DG, Haagmans BL, van der Veer B, van den Brink S, Wijsman L, Goderski G, Romette JL, Ellis J, Zambon M, Peiris M, Goossens H, Reusken C, Koopmans MP, Drosten C.
Journal Euro Surveill
Impact factor7.37
Citation count1206
Date of publishing2020 Jan
Reporting RT-qPCR E gene assay which, can be used as the first-line screening test for SARS-CoV-2 detection.
PMID 31992387
Title Detection of Covid 19 novel coronavirus (2019 -nCoV) by real-time RT-PCR
Author(s) nameCorman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DK, Bleicker T, Brünink S, Schneider J, Schmidt ML, Mulders DG, Haagmans BL, van der Veer B, van den Brink S, Wijsman L, Goderski G, Romette JL, Ellis J, Zambon M, Peiris M, Goossens H, Reusken C, Koopmans MP, Drosten C.
Journal Euro Surveill
Impact factor7.37
Citation count1206
Date of publishing2020 Jan