RT-PCR assay


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Reporting a conventional one-step RT-PCR assay, specific for the E gene of SARS-CoV-2, which can be used as a lower-cost alternative method for the detection of SARS-CoV-2 in remote places.
PMID 32767275
Title Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR
Author(s) nameDorlass EG, Monteiro CO, Viana AO, Soares CP, Machado RRG, Thomazelli LM, Araujo DB, Leal FB, Candido ED, Telezynski BL, Valério CA, Chalup VN, Mello R, Almeida FJ, Aguiar AS, Barrientos ACM, Sucupira C, De Paulis M, Sáfadi MAP, Silva DGBP, Sodré JJM, Soledade MP, Matos SF, Ferreira SR, Pinez CMN, Buonafine CP, Pieroni LNF, Malta FM, Santana RAF, Souza EC, Fock RA, Pinho JRR, Ferreira LCS, Botosso VF, Durigon EL, Oliveira DBL.
Journal Braz J Microbiol
Impact factor1.67
Citation count2
Date of publishing2020 Sep
Reporting a conventional two-step RT-PCR assay, specific for the E gene of SARS-CoV-2, which can be used as a lower-cost alternative method for the detection of SARS-CoV-2 in remote places.
PMID 32767275
Title Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR
Author(s) nameDorlass EG, Monteiro CO, Viana AO, Soares CP, Machado RRG, Thomazelli LM, Araujo DB, Leal FB, Candido ED, Telezynski BL, Valério CA, Chalup VN, Mello R, Almeida FJ, Aguiar AS, Barrientos ACM, Sucupira C, De Paulis M, Sáfadi MAP, Silva DGBP, Sodré JJM, Soledade MP, Matos SF, Ferreira SR, Pinez CMN, Buonafine CP, Pieroni LNF, Malta FM, Santana RAF, Souza EC, Fock RA, Pinho JRR, Ferreira LCS, Botosso VF, Durigon EL, Oliveira DBL.
Journal Braz J Microbiol
Impact factor1.67
Citation count2
Date of publishing2020 Sep
Reporting one step nested RT-PCR, specific for ORF1ab gene, which performs two successive rounds of amplification at low cost with greater flexibility.
PMID 32794453
Title One-step nested RT-PCR for COVID-19 detection: A flexible, locally developed test for SARS-CoV2 nucleic acid detection
Author(s) nameMeza-Robles C, Barajas-Saucedo CE, Tiburcio-Jimenez D, Mokay-Ramírez KA, Melnikov V, Rodriguez-Sanchez IP, Martinez-Fierro ML, Garza-Veloz I, Zaizar-Fregoso SA, Guzman-Esquivel J, Ramirez-Flores M, Newton-Sanchez OA, Espinoza-Gómez F, Delgado-Enciso OG, Centeno-Ramirez AS, Delgado-Enciso I.
Journal J Infect Dev Ctries
Impact factor1.26
Citation count4
Date of publishing2020 Jul 31
The implementation of Nested RT-PCR for the detection of COVID-19, based on SARS-CoV-2 Orf1a and S genes.
PMID 32074516
Title Development of genetic diagnostic methods for detection for Novel Coronavirus 2019 (nCoV-2019) in Japan
Author(s) nameShirato K, Nao N, Katano H, Takayama I, Saito S, Kato F, Katoh H, Sakata M, Nakatsu Y, Mori Y, Kageyama T, Matsuyama S, Takeda M.
Journal Jpn J Infect Dis
Impact factor1.11
Citation count72
Date of publishing2020 Jul 22
In this study, a clinically useful timeline of two diagnostic tests, reverse transcriptase-polymerase chain reaction and IgM and IgG enzyme-linked immunosorbent assay is reported, by using data from several published reports for detection of COVID-19. For symptomatic patients, viral RNA in the nasopharyngeal swab becomes detectable as early as day 1 of symptoms onset, peaks within the first week, declines by the third week, and subsequently becomes undetectable.
PMID 32374370
Title Interpreting diagnostic tests for SARS CoV-2
Author(s) nameSethuraman N, Jeremiah SS, Ryo A.
Journal JAMA
Impact factor14.78
Citation count209
Date of publishing2020 Jun 9