RT-LAMP assay


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Reporting a visual detection of SARS-CoV-2 by a rapid, simple, sensitive, and specific RT-LAMP method with the eight primer sets.
PMID 33098286
Title A sensitive reverse transcription loop mediated isothermal amplification assay for direct visual detection of SARS-CoV-2
Author(s) nameLau YL, Ismail IB, Izati Binti Mustapa N, Lai MY, Tuan Soh TS, Hassan AH, Peariasamy KM, Lee YL, Goh PP.
Journal Am J Trop Med Hyg
Impact factor2.25
Citation count1
Date of publishing2020 Dec
In this study, a colorimetric RT-LAMP method targeting the RdRp gene is developed for detecting SARS-CoV-2 within 60 min. The results showed high sensitivity and specificity of 95.74% and 99.95%, respectively.
PMID 33165486
Title Colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a visual diagnostic platform for the detection of the emerging coronavirus SARS-CoV-2
Author(s) nameNawattanapaiboon K , Pasomsub E , Prombun P , Wongbunmak A , Jenjitwanich A , Mahasupachai P , Vetcho P , Chayrach C , Manatjaroenlap N , Samphaongern C , Watthanachockchai T , Leedorkmai P , Manopwisedjaroen S , Akkarawongsapat R , Thitithanyanont A , Phanchana M , Panbangred W , Chauvatcharin S , Srikhirin T .
Journal Analyst
Impact factor3.98
Citation count1
Date of publishing2020 Nov 9
Evaluating the performance of CE-labeled variplexTM real-time SARS-CoV-2 RT-LAMP assay using extracted RNA in comparison with commercial Corman's LightMix E gene RT-PCR as reference.
PMID 32891938
Title Use of the variplex SARS-CoV-2 RT-LAMP as a rapid molecular assay to complement RT-PCR for COVID-19 diagnosis
Author(s) nameRödel J, Egerer R, Suleyman A, Sommer-Schmid B, Baier M, Henke A, Edel B, Löffler B.
Journal J Clin Virol
Impact factor2.95
Citation count4
Date of publishing2020 Nov
Evaluating the performance of CE-labeled variplexTM real-time SARS-CoV-2 RT-LAMP assay from direct respiratory samples, without RNA extraction, and comparing it with VIASURE and Allplex RT-PCR assays.
PMID 32891938
Title Use of the variplex SARS-CoV-2 RT-LAMP as a rapid molecular assay to complement RT-PCR for COVID-19 diagnosis
Author(s) nameRödel J, Egerer R, Suleyman A, Sommer-Schmid B, Baier M, Henke A, Edel B, Löffler B.
Journal J Clin Virol
Impact factor2.95
Citation count4
Date of publishing2020 Nov
Reporting a rapid, highly sensitive, reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay targeting the S gene for SARS-CoV-2 detection.
PMID 32848011
Title Development and Clinical Application of a Rapid and Sensitive Loop-Mediated Isothermal Amplification Test for SARS-CoV-2 Infection
Author(s) nameHu X, Deng Q, Li J, Chen J, Wang Z, Zhang X, Fang Z, Li H, Zhao Y, Yu P, Li W, Wang X, Li S, Zhang L, Hou T.
Journal mSphere
Impact factor4.28
Citation count3
Date of publishing2020 Aug 26
In this study, researchers tested a rapid, isothermal, colorimetric RT-LAMP protocol by using a specific primer set based on the N gene for detecting SARS-CoV-2. The assay detected SARS-CoV-2 RNA, with a detection limit corresponding to a CT value of 30 for RT-qPCR, with a sensitivity of 97.5% and a specificity of 99.7%.
PMID 32719001
Title A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples
Author(s) nameDao Thi VL, Herbst K, Boerner K, Meurer M, Kremer LP, Kirrmaier D, Freistaedter A, Papagiannidis D, Galmozzi C, Stanifer ML, Boulant S, Klein S, Chlanda P, Khalid D, Barreto Miranda I, Schnitzler P, Kräusslich HG, Knop M, Anders S.
Journal Sci Transl Med
Impact factor11.64
Citation count11
Date of publishing2020 Aug 12
In this study, researchers have developed a rapid, isothermal method, a swab-to-RT-LAMP assay that can be performed without RNA extraction, for detecting SARS-CoV-2. The assay had a specificity of 99.5% and sensitivity of 86% (for CT < 30).
PMID 32719001
Title A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples
Author(s) nameDao Thi VL, Herbst K, Boerner K, Meurer M, Kremer LP, Kirrmaier D, Freistaedter A, Papagiannidis D, Galmozzi C, Stanifer ML, Boulant S, Klein S, Chlanda P, Khalid D, Barreto Miranda I, Schnitzler P, Kräusslich HG, Knop M, Anders S.
Journal Sci Transl Med
Impact factor11.64
Citation count11
Date of publishing2020 Aug 12
Reporting a simple, rapid, and visual reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detection of SARS-CoV-2 by targeting the N gene. The results were observed using a real-time PCR machine or by a colorimetric change from red to yellow. The results showed 92.9% concordance with the RT-qPCR assay.
PMID 32325642
Title A Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2
Author(s) nameLu R, Wu X, Wan Z, Li Y, Jin X, Zhang C.
Journal Int J Mol Sci
Impact factor4.21
Citation count29
Date of publishing2020 Apr 18