Nested RT-PCR assay

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Key Findings
Reporting one step nested RT-PCR, specific for ORF1ab gene, which performs two successive rounds of amplification at low cost with greater flexibility.
PMID 32794453
Title One-step nested RT-PCR for COVID-19 detection: A flexible, locally developed test for SARS-CoV2 nucleic acid detection
Author(s) nameMeza-Robles C, Barajas-Saucedo CE, Tiburcio-Jimenez D, Mokay-Ramírez KA, Melnikov V, Rodriguez-Sanchez IP, Martinez-Fierro ML, Garza-Veloz I, Zaizar-Fregoso SA, Guzman-Esquivel J, Ramirez-Flores M, Newton-Sanchez OA, Espinoza-Gómez F, Delgado-Enciso OG, Centeno-Ramirez AS, Delgado-Enciso I.
Journal J Infect Dev Ctries
Impact factor1.26
Citation count4
Date of publishing2020 Jul 31
The implementation of Nested RT-PCR for the detection of COVID-19, based on SARS-CoV-2 Orf1a and S genes.
PMID 32074516
Title Development of genetic diagnostic methods for detection for Novel Coronavirus 2019 (nCoV-2019) in Japan
Author(s) nameShirato K, Nao N, Katano H, Takayama I, Saito S, Kato F, Katoh H, Sakata M, Nakatsu Y, Mori Y, Kageyama T, Matsuyama S, Takeda M.
Journal Jpn J Infect Dis
Impact factor1.11
Citation count72
Date of publishing2020 Jul 22