HC-FIA Assay

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Key Findings
Reporting an amplification-free, fluorescence-based diagnostic method, namely Hybrid Capture Fluorescence Immunoassay (HC-FIA), for diagnosing SARS-CoV-2. It is carried on a lateral flow strip and targets ORF1ab, E, and the N regions of the genome to detect SARS-CoV-2. The assay resulted in 100% and 99% sensitivities and specificities, respectively.
PMID 33273714
Title Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA
Author(s) nameWang D, He S, Wang X, Yan Y, Liu J, Wu S, Liu S, Lei Y, Chen M, Li L, Zhang J, Zhang L, Hu X, Zheng X, Bai J, Zhang Y, Zhang Y, Song M, Tang Y.
Journal Nat Biomed Eng
Impact factor
Citation count3
Date of publishing2020 Dec