Droplet digital PCR assay(ddPCR)


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In this research study, N- and E- gene multiplex (ddCoV_N and ddCoV_E) RT-ddPCR method is redesigned and optimized to detect SARS-CoV-2 from wastewater samples and compared with the standard nCoV_N2 and E_Sarbeco RT-qPCR assays.
PMID
Title Redesigning SARS-CoV-2 clinical RT-qPCR assays for wastewater RT-ddPCR
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Reporting one-step RT-dPCR (reverse transcription digital PCR ) method based on SARS-CoV-2 ORF1ab, N, and E gene for detecting SARS-CoV-2 from pharyngeal swab samples. RT dPCR uses droplets of samples with as low as 2 gene copies to diagnose COVID-19 positive.
PMID 33379001
Title Highly accurate and sensitive diagnostic detection of SARS-CoV-2 by digital PCR
Author(s) nameDong L, Zhou J, Niu C, Wang Q, Pan Y, Sheng S, Wang X, Zhang Y, Yang J, Liu M, Zhao Y, Zhang X, Zhu T, Peng T, Xie J, Gao Y, Wang D, Dai X, Fang X.
Journal Talanta
Impact factor
Citation count2
Date of publishing2021 Mar 1
Reporting a newly designed droplet digital PCR (ddPCR) assay, based on RNA-dependent RNA polymerase gene (RdRp) of SARS-CoV-2.
PMID 32898153
Title Detection and quantification of SARS-CoV-2 by droplet digital PCR in real-time PCR negative nasopharyngeal swabs from suspected COVID-19 patients
Author(s) nameAlteri C, Cento V, Antonello M, Colagrossi L, Merli M, Ughi N, Renica S, Matarazzo E, Di Ruscio F, Tartaglione L, Colombo J, Grimaldi C, Carta S, Nava A, Costabile V, Baiguera C, Campisi D, Fanti D, Vismara C, Fumagalli R, Scaglione F, Epis OM, Puoti M, Perno CF.
Journal PLoS One
Impact factor2.87
Citation count4
Date of publishing2020