Diagnostics


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In this research study, N- and E- gene multiplex (ddCoV_N and ddCoV_E) RT-ddPCR method is redesigned and optimized to detect SARS-CoV-2 from wastewater samples and compared with the standard nCoV_N2 and E_Sarbeco RT-qPCR assays.
PMID
Title Redesigning SARS-CoV-2 clinical RT-qPCR assays for wastewater RT-ddPCR
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The research focuses on the changes in serum SARS-CoV-2 specific IgM/IgG antibody levels in asymptomatic and reoccurring positive nucleic acid test (RPNAT) group patients. The IgM levels of the RPNAT group at the time of discharge were higher than those Non-RPNAT group.
PMID 32808808
Title Changes in serum virus specific IgM/IgG antibody in asymptomatic and discharged patients with reoccuring positive COVID-19 nucleic acid test
Author(s) nameLiu J, Lian R, Zhang G, Hou B, Wang C, Dong J, Yang L, Wang J, Dai S, Chen L, Zhang G, Lu X, Ye T.
Journal Ann Med
Impact factor3.01
Citation count3
Date of publishing2021 Dec
Reporting an AI-based diagnosis method that integrates Self-supervised Learning as a plugin module into a Multi-task Convolutional Neural Network for the automatic diagnosis of COVID-19 based on both CT and X-ray datasets.
PMID 33518812
Title Multi-task contrastive learning for automatic CT and X-ray diagnosis of COVID-19
Author(s) nameLi J, Zhao G, Tao Y, Zhai P, Chen H, He H, Cai T.
Journal Pattern Recognit
Impact factor7.35
Citation count1
Date of publishing2021 Jun
Reporting one-step RT-dPCR (reverse transcription digital PCR ) method based on SARS-CoV-2 ORF1ab, N, and E gene for detecting SARS-CoV-2 from pharyngeal swab samples. RT dPCR uses droplets of samples with as low as 2 gene copies to diagnose COVID-19 positive.
PMID 33379001
Title Highly accurate and sensitive diagnostic detection of SARS-CoV-2 by digital PCR
Author(s) nameDong L, Zhou J, Niu C, Wang Q, Pan Y, Sheng S, Wang X, Zhang Y, Yang J, Liu M, Zhao Y, Zhang X, Zhu T, Peng T, Xie J, Gao Y, Wang D, Dai X, Fang X.
Journal Talanta
Impact factor
Citation count2
Date of publishing2021 Mar 1
Immunosensor based quantification of the ultralow concentration of cytokines (IL-6, TNF-alpha, etc.) present in blood or respiratory samples to monitor local and systemic inflammation.
PMID 33519090
Title Paper biosensors for detecting elevated IL-6 levels in blood and respiratory samples from COVID-19 patients
Author(s) nameAdrover-Jaume C, Alba-Patiño A, Clemente A, Santopolo G, Vaquer A, Russell SM, Barón E, González Del Campo MDM, Ferrer JM, Berman-Riu M, García-Gasalla M, Aranda M, Borges M, de la Rica R.
Journal Sens Actuators B Chem
Impact factor
Citation count1
Date of publishing2021 Mar 1
A new nanotechnology-based PCR-assisted colorimetric SARS-CoV-2 detection assay works on the principle of 5'-exonuclease activity of polymerase and linker-based single-component assembly of gold nanoparticle-core spherical nucleic acids. The positive and negative viral samples produce red and purple colors in the post-PCR colorimetric test, respectively.
PMID 33012989
Title Conventional PCR Assisted Single-Component Assembly of Spherical Nucleic Acids for Simple Colorimetric Detection of SARS-CoV-2
Author(s) nameKarami A, Hasani M, Azizi Jalilian F, Ezati R.
Journal Sens Actuators B Chem
Impact factor
Citation count2
Date of publishing2021 Feb 1
Detection of SARS CoV-2 IgM, and IgA antibodies from Sera samples using Luminex bead-based assay by targeting SARS-CoV-2 spike and nucleocapsid antigens. The neutralizing and binding IgG, IgA, and IgM antibody levels were higher for severe than mild cases in the early convalescent phase (<6 weeks).
PMID 33227340
Title Evaluating SARS-CoV-2 spike and nucleocapsid proteins as targets for antibody detection in severe and mild COVID-19 cases using a Luminex bead-based assay
Author(s) nameMariën J, Ceulemans A, Michiels J, Heyndrickx L, Kerkhof K, Foque N, Widdowson MA, Mortgat L, Duysburgh E, Desombere I, Jansens H, Van Esbroeck M, Ariën KK.
Journal J Virol Methods
Impact factor1.76
Citation count1
Date of publishing2021 Feb
Compared to the SARS-CoV-2 RT-PCR assay, the SARS-CoV-2 Rapid Antigen Test (Roche, Switzerland) showed less sensitivity and specificity.
PMID 33227341
Title Comparision of the SARS-CoV-2 rapid antigen test to real star SARS-CoV-2 RT-PCR kit
Author(s) nameKrüttgen A, Cornelissen CG, Dreher M, Hornef MW, Imöhl M, Kleines M.
Journal J Virol Methods
Impact factor1.76
Citation count4
Date of publishing2021 Feb
A chemiluminescent assay that detects IgG antibodies against nucleocapsid gene with utmost specificity
PMID 32710669
Title Validation and performance comparision of three SARS-CoV-2 antibody assays
Author(s) namePaiva KJ, Grisson RD, Chan PA, Huard RC, Caliendo AM, Lonks JR, King E, Tang EW, Pytel-Parenteau DL, Nam GH, Yakirevich E, Lu S.
Journal J Med Virol
Impact factor2.07
Citation count4
Date of publishing2021 Feb
A total antibody test that detects IgG and IgM concentrations against the spike protein in a single cartridge with maximum specificity
PMID 32710669
Title Validation and performance comparision of three SARS-CoV-2 antibody assays
Author(s) namePaiva KJ, Grisson RD, Chan PA, Huard RC, Caliendo AM, Lonks JR, King E, Tang EW, Pytel-Parenteau DL, Nam GH, Yakirevich E, Lu S.
Journal J Med Virol
Impact factor2.07
Citation count4
Date of publishing2021 Feb
A lateral flow assay that detects IgG and IgM antibody concentration against spike protein in two different cartridges with just maximum specificity
PMID 32710669
Title Validation and performance comparision of three SARS-CoV-2 antibody assays
Author(s) namePaiva KJ, Grisson RD, Chan PA, Huard RC, Caliendo AM, Lonks JR, King E, Tang EW, Pytel-Parenteau DL, Nam GH, Yakirevich E, Lu S.
Journal J Med Virol
Impact factor2.07
Citation count4
Date of publishing2021 Feb
In this study, the Rapid Antigen detection kit, STANDARD F COVID-19 Ag FIA (FIA), a fluorescent immunoassay, was evaluated for the rapid detection of SARS-CoV-2 nucleoprotein in PCR-positive and PCR-negative swab samples. The kit produced false-negative results, with overall clinical sensitivity and specificity of 45.4% and 97.8%, respectively.
PMID 33452927
Title Evaluation of two rapid antigen tests to detect SARS-CoV-2 in a hospital setting
Author(s) nameOsterman A, Baldauf HM, Eletreby M, Wettengel JM, Afridi SQ, Fuchs T, Holzmann E, Maier A, Döring J, Grzimek-Koschewa N, Muenchhoff M, Protzer U, Kaderali L, Keppler OT.
Journal Med Microbiol Immunol
Impact factor
Citation count1
Date of publishing2021 Jan 16
In this study, the Rapid Antigen detection kit, Roche SARS-CoV-2 Rapid Antigen Test (RAT), a chromatographic immunoassay, was evaluated for the rapid detection of SARS-CoV-2 N protein in PCR-positive and PCR-negative swab samples. The kit produced false-negative results, with overall clinical sensitivity and specificity of 50.3% and 97.7%, respectively.
PMID 33452927
Title Evaluation of two rapid antigen tests to detect SARS-CoV-2 in a hospital setting
Author(s) nameOsterman A, Baldauf HM, Eletreby M, Wettengel JM, Afridi SQ, Fuchs T, Holzmann E, Maier A, Döring J, Grzimek-Koschewa N, Muenchhoff M, Protzer U, Kaderali L, Keppler OT.
Journal Med Microbiol Immunol
Impact factor
Citation count1
Date of publishing2021 Jan 16
In this study, the diagnostic performance of the structured approach of chest radiograph was evaluated and compared with RT-PCR. The study revealed that positive chest radiography had a high likelihood of predicting a positive RT-PCR. The accuracy of baseline CXR in the diagnosis of COVID-19 is moderate to good, with high specificity and low sensitivity.
PMID 33246271
Title Structural reporting in portable chest radiographs : An essential tool in the diagnosis of COVID-19
Author(s) nameYates A, Dempsey PJ, Vencken S, MacMahon PJ, Hutchinson BD.
Journal Eur J Radiol
Impact factor3.11
Citation count1
Date of publishing2021 Jan
Reporting a CRISPR Cas12a-Mediated RT-LAMP assay, highly specific for the detection of E and N gene of SARS-CoV-2.
PMID 33238709
Title Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology
Author(s) namePang B, Xu J, Liu Y, Peng H, Feng W, Cao Y, Wu J, Xiao H, Pabbaraju K, Tipples G, Joyce MA, Saffran HA, Tyrrell DL, Zhang H, Le XC.
Journal Anal Chem
Impact factor6.37
Citation count1
Date of publishing2020 Dec 15
Reporting a visual detection of SARS-CoV-2 by a rapid, simple, sensitive, and specific RT-LAMP method with the eight primer sets.
PMID 33098286
Title A sensitive reverse transcription loop mediated isothermal amplification assay for direct visual detection of SARS-CoV-2
Author(s) nameLau YL, Ismail IB, Izati Binti Mustapa N, Lai MY, Tuan Soh TS, Hassan AH, Peariasamy KM, Lee YL, Goh PP.
Journal Am J Trop Med Hyg
Impact factor2.25
Citation count1
Date of publishing2020 Dec
Reporting SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) method to detect SARS-CoV-2, which relies on the RT-RPA method to amplify viral gene segments, followed by CRISPR-Cas13a-mediated detection of amplified genes. The assay was 100% specific, and 96% sensitive with the fluorescence readout, and 88% sensitive with the lateral-flow readout.
PMID 32848209
Title Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA
Author(s) namePatchsung M, Jantarug K, Pattama A, Aphicho K, Suraritdechachai S, Meesawat P, Sappakhaw K, Leelahakorn N, Ruenkam T, Wongsatit T, Athipanyasilp N, Eiamthong B, Lakkanasirorat B, Phoodokmai T, Niljianskul N, Pakotiprapha D, Chanarat S, Homchan A, Tinikul R, Kamutira P, Phiwkaow K, Soithongcharoen S, Kantiwiriyawanitch C, Pongsupasa V, Trisrivirat D, Jaroensuk J, Wongnate T, Maenpuen S, Chaiyen P, Kamnerdnakta S, Swangsri J, Chuthapisith S, Sirivatanauksorn Y, Chaimayo C, Sutthent R, Kantakamalakul W, Joung J, Ladha A, Jin X, Gootenberg JS, Abudayyeh OO, Zhang F, Horthongkham N, Uttamapinant C.
Journal Nat Biomed Eng
Impact factor
Citation count16
Date of publishing2020 Dec
Reporting a new, fast detection method, NanoPCR assay specific for the N1 and N2 gene of SARS-CoV-2 based on plasmonic RT-PCR with in-situ fluorescence signal detection.
PMID 33273713
Title Fast detection of SARS CoV-2 RNA via the integration of plasmonic thermocycling and fluoroscence detection in a portable device
Author(s) nameCheong J, Yu H, Lee CY, Lee JU, Choi HJ, Lee JH, Lee H, Cheon J.
Journal Nat Biomed Eng
Impact factor
Citation count2
Date of publishing2020 Dec
Reporting a highly sensitive and specific one-pot fluorescence-based detection assay, SENSR assay (sensitive splint-based one-pot isothermal RNA ) based on RdRp gene to detect SARS-CoV-2.
PMID 32948855
Title Sensitive fluorescence detection of SARS-CoV-2 RNA in clinical samples via one-pot isothermal ligation and transcription
Author(s) nameWoo CH, Jang S, Shin G, Jung GY, Lee JW.
Journal Nat Biomed Eng
Impact factor
Citation count1
Date of publishing2020 Dec
Reporting an amplification-free, fluorescence-based diagnostic method, namely Hybrid Capture Fluorescence Immunoassay (HC-FIA), for diagnosing SARS-CoV-2. It is carried on a lateral flow strip and targets ORF1ab, E, and the N regions of the genome to detect SARS-CoV-2. The assay resulted in 100% and 99% sensitivities and specificities, respectively.
PMID 33273714
Title Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA
Author(s) nameWang D, He S, Wang X, Yan Y, Liu J, Wu S, Liu S, Lei Y, Chen M, Li L, Zhang J, Zhang L, Hu X, Zheng X, Bai J, Zhang Y, Zhang Y, Song M, Tang Y.
Journal Nat Biomed Eng
Impact factor
Citation count3
Date of publishing2020 Dec
In this study, a colorimetric RT-LAMP method targeting the RdRp gene is developed for detecting SARS-CoV-2 within 60 min. The results showed high sensitivity and specificity of 95.74% and 99.95%, respectively.
PMID 33165486
Title Colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a visual diagnostic platform for the detection of the emerging coronavirus SARS-CoV-2
Author(s) nameNawattanapaiboon K , Pasomsub E , Prombun P , Wongbunmak A , Jenjitwanich A , Mahasupachai P , Vetcho P , Chayrach C , Manatjaroenlap N , Samphaongern C , Watthanachockchai T , Leedorkmai P , Manopwisedjaroen S , Akkarawongsapat R , Thitithanyanont A , Phanchana M , Panbangred W , Chauvatcharin S , Srikhirin T .
Journal Analyst
Impact factor3.98
Citation count1
Date of publishing2020 Nov 9
Reporting an optimized protocol serum-based enzyme-linked immunosorbent assay (ELISA) to detect antibodies against SARS-CoV-2 in saliva samples. The assay gave a sensitivity of 84.2% and a specificity of 100% in a general symptomatic population.
PMID 33257702
Title ELISA detection of SARS-CoV-2 antibodies in saliva
Author(s) nameMacMullan MA, Ibrayeva A, Trettner K, Deming L, Das S, Tran F, Moreno JR, Casian JG, Chellamuthu P, Kraft J, Kozak K, Turner FE, Slepnev VI, Le Page LM.
Journal Sci Rep
Impact factor4.12
Citation count1
Date of publishing2020 Nov 30
An alternative method for the detection of SARS-CoV-2 directly from clinical specimens by application of direct Oxford Nanopore long-read third-generation metatranscriptomic and metagenomic sequencing technology. mNGS provided valuable information about the patients' respiratory microbiome and its relationship to coinfections.
PMID 33219095
Title Metagenomic Next-Generation Sequencing of Nasopharyngeal Specimens Collected from Confirmed and Suspect COVID-19 Patients
Author(s) nameMostafa HH, Fissel JA, Fanelli B, Bergman Y, Gniazdowski V, Dadlani M, Carroll KC, Colwell RR, Simner PJ.
Journal mBio
Impact factor6.5
Citation count2
Date of publishing2020 Nov 20
Reporting a novel nsp1 real-time RT-PCR assay for the detection of SARS-CoV-2, based on nanopore sequencing.
PMID 32501535
Title Identification of nsp1 gene as the target of SARS-CoV-2 real time RT-PCR using nanopore whole genome sequencing
Author(s) nameChan WM, Ip JD, Chu AW, Yip CC, Lo LS, Chan KH, Ng AC, Poon RW, To WK, Tsang OT, Leung WS, Kwan MY, Chua GT, Chung TW, Hung IF, Kok KH, Cheng VC, Chan JF, Yuen KY, To KK.
Journal J Med Virol
Impact factor2.07
Citation count5
Date of publishing2020 Nov
Evaluating the performance of CE-labeled variplexTM real-time SARS-CoV-2 RT-LAMP assay using extracted RNA in comparison with commercial Corman's LightMix E gene RT-PCR as reference.
PMID 32891938
Title Use of the variplex SARS-CoV-2 RT-LAMP as a rapid molecular assay to complement RT-PCR for COVID-19 diagnosis
Author(s) nameRödel J, Egerer R, Suleyman A, Sommer-Schmid B, Baier M, Henke A, Edel B, Löffler B.
Journal J Clin Virol
Impact factor2.95
Citation count4
Date of publishing2020 Nov