CRISPR based assay


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Key Findings
Reporting a CRISPR Cas12a-Mediated RT-LAMP assay, highly specific for the detection of E and N gene of SARS-CoV-2.
PMID 33238709
Title Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR technology
Author(s) namePang B, Xu J, Liu Y, Peng H, Feng W, Cao Y, Wu J, Xiao H, Pabbaraju K, Tipples G, Joyce MA, Saffran HA, Tyrrell DL, Zhang H, Le XC.
Journal Anal Chem
Impact factor6.37
Citation count1
Date of publishing2020 Dec 15
Reporting SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) method to detect SARS-CoV-2, which relies on the RT-RPA method to amplify viral gene segments, followed by CRISPR-Cas13a-mediated detection of amplified genes. The assay was 100% specific, and 96% sensitive with the fluorescence readout, and 88% sensitive with the lateral-flow readout.
PMID 32848209
Title Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA
Author(s) namePatchsung M, Jantarug K, Pattama A, Aphicho K, Suraritdechachai S, Meesawat P, Sappakhaw K, Leelahakorn N, Ruenkam T, Wongsatit T, Athipanyasilp N, Eiamthong B, Lakkanasirorat B, Phoodokmai T, Niljianskul N, Pakotiprapha D, Chanarat S, Homchan A, Tinikul R, Kamutira P, Phiwkaow K, Soithongcharoen S, Kantiwiriyawanitch C, Pongsupasa V, Trisrivirat D, Jaroensuk J, Wongnate T, Maenpuen S, Chaiyen P, Kamnerdnakta S, Swangsri J, Chuthapisith S, Sirivatanauksorn Y, Chaimayo C, Sutthent R, Kantakamalakul W, Joung J, Ladha A, Jin X, Gootenberg JS, Abudayyeh OO, Zhang F, Horthongkham N, Uttamapinant C.
Journal Nat Biomed Eng
Impact factor
Citation count16
Date of publishing2020 Dec
Reporting a simple STOP (SHERLOCK testing in one pot assay) diagnostic method for the detection of the SARS-CoV-2 N gene. This method combines viral RNA extraction with isothermal amplification and CRISPR-mediated detection technology.
PMID 32937062
Title Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing
Author(s) nameJoung J, Ladha A, Saito M, Kim NG, Woolley AE, Segel M, Barretto RPJ, Ranu A, Macrae RK, Faure G, Ioannidi EI, Krajeski RN, Bruneau R, Huang MW, Yu XG, Li JZ, Walker BD, Hung DT, Greninger AL, Jerome KR, Gootenberg JS, Abudayyeh OO, Zhang F.
Journal N Engl J Med
Impact factor37.91
Citation count16
Date of publishing2020 Oct 8
In this study, iSCAN assay (in vitro Specific CRISPR-based Assay for Nucleic acids) is developed, involving RT-LAMP coupled with CRISPR-Cas12, for rapid, accurate, specific, sensitive detection of COVID-19.
PMID 32822689
Title iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module
Author(s) nameAli Z, Aman R, Mahas A, Rao GS, Tehseen M, Marsic T, Salunke R, Subudhi AK, Hala SM, Hamdan SM, Pain A, Alofi FS, Alsomali A, Hashem AM, Khogeer A, Almontashiri NAM, Abedalthagafi M, Hassan N, Mahfouz MM.
Journal Virus Res
Impact factor2.6
Citation count11
Date of publishing2020 Oct 15
Reporting a one-step RT-AIOD-CRISPR assay based on a unique dual CRISPR-Cas12a detection method for the rapid detection of SARS-COV-2.
PMID 32948757
Title Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay
Author(s) nameDing X, Yin K, Li Z, Lalla RV, Ballesteros E, Sfeir MM, Liu C.
Journal Nat Commun
Impact factor11.8
Citation count13
Date of publishing2020 Sep 18
In this study, a rapid, accurate CRISPR-Cas12-based lateral flow assay is developed to detect SARS-CoV-2 from clinical samples. The CRISPR-based DETECTR technology provides a faster alternative to the US CDC RT-qPCR assay, with 95% positive predictive agreement and 100% negative predictive agreement.
PMID 32300245
Title CRISPR-Cas12-based detection of SARS-CoV-2
Author(s) nameBroughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao X, Streithorst JA, Granados A, Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu E, Gu W, Miller S, Pan CY, Guevara H, Wadford DA, Chen JS, Chiu CY.
Journal Nat Biotechnol
Impact factor
Citation count155
Date of publishing2020 Jul
In this study, a CRISPR-assisted detection protocol (CASdetec) is developed and optimized for SARS-CoV-2 detection. The protocol includes virus handling, nucleic acid amplification by RT-RAA, and CRISPR-based detection with a blue LED for positive samples. This assay can detect the virus with at least 1 10^4 copies/mL, with no cross-reactivity with the other human coronaviruses.
PMID 32435508
Title SARS-CoV-2 detection with CRISPR diagnostics
Author(s) nameGuo L, Sun X, Wang X, Liang C, Jiang H, Gao Q, Dai M, Qu B, Fang S, Mao Y, Chen Y, Feng G, Gu Q, Wang RR, Zhou Q, Li W.
Journal Cell Discov
Impact factor4.65
Citation count18
Date of publishing2020