Antibody assays


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Key Findings
The research focuses on the changes in serum SARS-CoV-2 specific IgM/IgG antibody levels in asymptomatic and reoccurring positive nucleic acid test (RPNAT) group patients. The IgM levels of the RPNAT group at the time of discharge were higher than those Non-RPNAT group.
PMID 32808808
Title Changes in serum virus specific IgM/IgG antibody in asymptomatic and discharged patients with reoccuring positive COVID-19 nucleic acid test
Author(s) nameLiu J, Lian R, Zhang G, Hou B, Wang C, Dong J, Yang L, Wang J, Dai S, Chen L, Zhang G, Lu X, Ye T.
Journal Ann Med
Impact factor3.01
Citation count3
Date of publishing2021 Dec
A total antibody test that detects IgG and IgM concentrations against the spike protein in a single cartridge with maximum specificity
PMID 32710669
Title Validation and performance comparision of three SARS-CoV-2 antibody assays
Author(s) namePaiva KJ, Grisson RD, Chan PA, Huard RC, Caliendo AM, Lonks JR, King E, Tang EW, Pytel-Parenteau DL, Nam GH, Yakirevich E, Lu S.
Journal J Med Virol
Impact factor2.07
Citation count4
Date of publishing2021 Feb
A lateral flow assay that detects IgG and IgM antibody concentration against spike protein in two different cartridges with just maximum specificity
PMID 32710669
Title Validation and performance comparision of three SARS-CoV-2 antibody assays
Author(s) namePaiva KJ, Grisson RD, Chan PA, Huard RC, Caliendo AM, Lonks JR, King E, Tang EW, Pytel-Parenteau DL, Nam GH, Yakirevich E, Lu S.
Journal J Med Virol
Impact factor2.07
Citation count4
Date of publishing2021 Feb
A chemiluminescent assay that detects IgG antibodies against nucleocapsid gene with utmost specificity
PMID 32710669
Title Validation and performance comparision of three SARS-CoV-2 antibody assays
Author(s) namePaiva KJ, Grisson RD, Chan PA, Huard RC, Caliendo AM, Lonks JR, King E, Tang EW, Pytel-Parenteau DL, Nam GH, Yakirevich E, Lu S.
Journal J Med Virol
Impact factor2.07
Citation count4
Date of publishing2021 Feb
Detection of SARS CoV-2 IgM, and IgA antibodies from Sera samples using Luminex bead-based assay by targeting SARS-CoV-2 spike and nucleocapsid antigens. The neutralizing and binding IgG, IgA, and IgM antibody levels were higher for severe than mild cases in the early convalescent phase (<6 weeks).
PMID 33227340
Title Evaluating SARS-CoV-2 spike and nucleocapsid proteins as targets for antibody detection in severe and mild COVID-19 cases using a Luminex bead-based assay
Author(s) nameMariën J, Ceulemans A, Michiels J, Heyndrickx L, Kerkhof K, Foque N, Widdowson MA, Mortgat L, Duysburgh E, Desombere I, Jansens H, Van Esbroeck M, Ariën KK.
Journal J Virol Methods
Impact factor1.76
Citation count1
Date of publishing2021 Feb
Reporting an optimized protocol serum-based enzyme-linked immunosorbent assay (ELISA) to detect antibodies against SARS-CoV-2 in saliva samples. The assay gave a sensitivity of 84.2% and a specificity of 100% in a general symptomatic population.
PMID 33257702
Title ELISA detection of SARS-CoV-2 antibodies in saliva
Author(s) nameMacMullan MA, Ibrayeva A, Trettner K, Deming L, Das S, Tran F, Moreno JR, Casian JG, Chellamuthu P, Kraft J, Kozak K, Turner FE, Slepnev VI, Le Page LM.
Journal Sci Rep
Impact factor4.12
Citation count1
Date of publishing2020 Nov 30
Reporting SARS-CoV-2 IgG-IgM combined antibody test kit, which is based on lateral flow immune assay, used for the rapid detection of the anti-SARS-CoV-2 IgM and anti-SARS-CoV-2 IgG
PMID 32104917
Title Development and clinical application of a rapid IgM-IgG combined antibody test for SARS-CoV-2 infection diagnosis
Author(s) nameLi Z, Yi Y, Luo X, Xiong N, Liu Y, Li S, Sun R, Wang Y, Hu B, Chen W, Zhang Y, Wang J, Huang B, Lin Y, Yang J, Cai W, Wang X, Cheng J, Chen Z, Sun K, Pan W, Zhan Z, Chen L, Ye F.
Journal J Med Virol
Impact factor2.07
Citation count332
Date of publishing2020 Sep
Reporting an indirect enzyme-linked immunosorbent assay (ELISA) protocol to detect IgA antibody against SARS-CoV-2 rNPs from plasma samples collected at different time points after post-onset of COVID-19 symptom. IgA antibody was detected 5 (IQR, 3-6) days after symptom onset.
PMID 32198501
Title Profiling early humoral response to diagnose novel coronavirus disease (COVID-19)
Author(s) nameGuo L, Ren L, Yang S, Xiao M, Chang D, Yang F, Dela Cruz CS, Wang Y, Wu C, Xiao Y, Zhang L, Han L, Dang S, Xu Y, Yang QW, Xu SY, Zhu HD, Xu YC, Jin Q, Sharma L, Wang L, Wang J.
Journal Clin Infect Dis
Impact factor7.71
Citation count295
Date of publishing2020 Jul 28
Reporting an indirect enzyme-linked immunosorbent assay (ELISA) protocol to detect IgM antibody against SARS-CoV-2 rNPs from plasma samples collected at different time points after post-onset of COVID-19 symptom. IgM antibody was detected 5 (IQR, 3-6) days after symptom onset.
PMID 32198501
Title Profiling early humoral response to diagnose novel coronavirus disease (COVID-19)
Author(s) nameGuo L, Ren L, Yang S, Xiao M, Chang D, Yang F, Dela Cruz CS, Wang Y, Wu C, Xiao Y, Zhang L, Han L, Dang S, Xu Y, Yang QW, Xu SY, Zhu HD, Xu YC, Jin Q, Sharma L, Wang L, Wang J.
Journal Clin Infect Dis
Impact factor7.71
Citation count295
Date of publishing2020 Jul 28
Reporting an indirect enzyme-linked immunosorbent assay (ELISA) protocol to detect IgG antibody against SARS-CoV-2 rNPs from plasma samples collected at different time points after post-onset of COVID-19 symptom. IgG antibody was detected 14 (IQR, 10-18) days after symptom onset.
PMID 32198501
Title Profiling early humoral response to diagnose novel coronavirus disease (COVID-19)
Author(s) nameGuo L, Ren L, Yang S, Xiao M, Chang D, Yang F, Dela Cruz CS, Wang Y, Wu C, Xiao Y, Zhang L, Han L, Dang S, Xu Y, Yang QW, Xu SY, Zhu HD, Xu YC, Jin Q, Sharma L, Wang L, Wang J.
Journal Clin Infect Dis
Impact factor7.71
Citation count295
Date of publishing2020 Jul 28
IgM ELISA test showed better sensitivity than a qPCR-based method alone. The positive detection rate increased when combined with the PCR method. Thus, IgM ELISA resulted better for the early diagnosis of COVID-19 patients.
PMID 32198501
Title Profiling early humoral response to diagnose novel coronavirus disease (COVID-19)
Author(s) nameGuo L, Ren L, Yang S, Xiao M, Chang D, Yang F, Dela Cruz CS, Wang Y, Wu C, Xiao Y, Zhang L, Han L, Dang S, Xu Y, Yang QW, Xu SY, Zhu HD, Xu YC, Jin Q, Sharma L, Wang L, Wang J.
Journal Clin Infect Dis
Impact factor7.71
Citation count295
Date of publishing2020 Jul 28
In this study, a serological method, an ELISA test, is developed to detect seroconversion upon SARS-CoV-2 infection. The test detects different antibody types in serum and plasma samples of COVID-19 patients.
PMID 32398876
Title A serological assay to detect SARS-CoV-2 seroconversion in humans
Author(s) nameAmanat F, Stadlbauer D, Strohmeier S, Nguyen THO, Chromikova V, McMahon M, Jiang K, Arunkumar GA, Jurczyszak D, Polanco J, Bermudez-Gonzalez M, Kleiner G, Aydillo T, Miorin L, Fierer DS, Lugo LA, Kojic EM, Stoever J, Liu STH, Cunningham-Rundles C, Felgner PL, Moran T, García-Sastre A, Caplivski D, Cheng AC, Kedzierska K, Vapalahti O, Hepojoki JM, Simon V, Krammer F.
Journal Nat Med
Impact factor22.66
Citation count263
Date of publishing2020 Jul
In this study, a clinically useful timeline of two diagnostic tests, reverse transcriptase-polymerase chain reaction and IgM and IgG enzyme-linked immunosorbent assay is reported, by using data from several published reports for detection of COVID-19. The study revealed that the nucleocapsid and RBD-S antigens showed a high sensitivity for detecting the infection. The serological assay performed two weeks later of symptoms onset had more diagnostic accuracy.
PMID 32374370
Title Interpreting diagnostic tests for SARS CoV-2
Author(s) nameSethuraman N, Jeremiah SS, Ryo A.
Journal JAMA
Impact factor14.78
Citation count209
Date of publishing2020 Jun 9